X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels

被引：1

作者：

Chen, Qiang ^{[1
]}

Xu, Yan ^{[1
]}

Tang, Pei ^{[1
]}

机构：

[1] Univ Pittsburgh, Sch Med, Pittsburgh, PA 15260 USA

来源：

CHEMICAL AND BIOCHEMICAL APPROACHES FOR THE STUDY OF ANESTHETIC FUNCTION, PT B | 2018年 / 603卷

关键词：

NICOTINIC ACETYLCHOLINE-RECEPTOR; CRYSTAL-STRUCTURES; TRANSMEMBRANE DOMAIN; STRUCTURAL BASIS; MECHANISM; CRYSTALLIZATION; PORE; NMR; INHIBITION; ISOFLURANE;

D O I：

10.1016/bs.mie.2018.01.017

中图分类号：

R614 [麻醉学];

学科分类号：

100217 ;

摘要：

X-ray crystallography is a powerful tool in structural biology and can offer insight into structured-based understanding of general anesthetic action on various relevant molecular targets, including pentameric ligand-gated ion channels (pLGICs). In this chapter, we outline the procedures for expression and purification of pLGICs. Optimization of crystallization conditions, especially to achieve high-resolution structures of pLGICs bound with general anesthetics, is also presented. Case studies of pLGICs bound with the volatile general anesthetic isoflurane, 2-bromoethanol, and the intravenous general anesthetic ketamine are revisited.

引用

页码：21 / 47

页数：27

共 91 条

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