Gum tragacanth modified nano-hydroxyapatite: An angiogenic- osteogenic biomaterial for bone tissue engineering

被引:13
作者
Dixit, Krishna [1 ]
Kulanthaivel, Senthilguru [2 ]
Agarwal, Tarun [3 ]
Pal, Kunal [4 ]
Giri, Supratim [5 ]
Maiti, T. K. [3 ]
Banerjee, Indranil [6 ]
机构
[1] Indian Inst Technol Kharagpur, Sch Med Sci & Technol, Kharagpur 721302, W Bengal, India
[2] Indian Inst Technol Delhi, Dept Biochem Engn & Biotechnol, New Delhi 110016, India
[3] Indian Inst Technol Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India
[4] Natl Inst Technol, Dept Biotechnol & Med Engn, Rourkela 769008, Odisha, India
[5] Natl Inst Technol, Dept Chem, Rourkela 769008, Odisha, India
[6] Indian Inst Technol Jodhpur, Dept Biosci & Bioengn, Jodhpur, Rajasthan, India
关键词
Hydroxyapatite; Biofunctionalization; VEGF; Osteogenic; Gum tragacanth; Bone tissue engineering; IN-VITRO; EXTRACELLULAR-MATRIX; PROTEIN ADSORPTION; CONTROLLED-RELEASE; REGENERATION; CALCIUM; DIFFERENTIATION; VASCULARIZATION; ANTIBACTERIAL; OSTEOBLASTS;
D O I
10.1016/j.ceramint.2022.02.002
中图分类号
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Bone tissue engineering (BTE) is an evolving domain and provides encouraging solution to address the current demands in the orthopaedic healthcare system. However, even a well-exploited biomaterial such as synthetic hydroxyapatite (nHAp) fails to induce ideal bone regeneration in the complex in vivo conditions owing to its poor angiogenic potential. Hence, in addition to the osteogenic property, designing smart biomaterials with intrinsic angiogenic property is key for the success of bone tissue engineering implants. Recently, gum tragacanth (TG) has been testified to imbibe both angiogenic and osteogenic characteristics. In this context, TG functionalized nano-hydroxyapatite (TG-HAp) would be an ideal angiogenic-osteogenic material for BTE application. Here, we have reported the biofunctionalization of nHAp with TG through a silanized nHAp intermediate, and its osteogenic and angiogenic properties. X-ray diffraction (XRD) revealed the crystalline apatite phase of HAp. Biofunctionalization of nHAP with TG was confirmed both qualitatively and quantitatively by FTIR and TGA analysis respectively. TEM analysis confirmed the formation of needle shaped nanoparticles having size range (100-300 nm). Biofunctionalization lead to a change in zetapotential of nHAp from - 8.1 mV to 0.1 mV. The existence of multi-layered gum tragacanth on nHAp was confirmed by BET analysis. TG-HAp was found osteogenic when tested invitro using MG-63, (human osteoblast cells) and hMSCs, (human mesenchymal stem cells). Further, TG-HAp was found to increase the cellular VEGF expression in MG-63. Angiogenic property of TG-HAp was confirmed by in vitro tube formation of human umbilical vein endothelial cells (HUVECs). The study altogether indicates about the angiogenic and osteogenic potential of synthesized TG-HAp, which may be used as a new biomaterial in bone tissue engineering.
引用
收藏
页码:14672 / 14683
页数:12
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