MAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Influence on Metal Exclusion/Extrusion Mechanisms and Photosynthesis

被引:14
作者
Celis-Pla, Paula S. M. [1 ]
Rodriguez-Rojas, Fernanda [1 ]
Mendez, Lorena [1 ]
Moenne, Fabiola [1 ]
Munoz, Pamela T. [1 ,2 ,3 ]
Gabriela Lobos, M. [4 ]
Diaz, Patricia [4 ]
Luis Sanchez-Lizaso, Jose [5 ]
Brown, Murray T. [6 ]
Moenne, Alejandra [7 ]
Saez, Claudio A. [1 ,8 ]
机构
[1] Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar 2520000, Chile
[2] Univ Playa Ancha, Fac Ciencias Nat & Exactas, Ciencias Ambientales, Valparaiso 2340000, Chile
[3] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, Ciencias Mar & Biol Aplicada, E-03080 Alicante, Spain
[4] Univ Valparaiso, Inst Quim & Bioquim, Lab Environm & Analyt Chem, Fac Ciencias, Valparaiso 234000, Chile
[5] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, E-03080 Alicante, Spain
[6] Univ Plymouth, Sch Biol & Marine Sci, Plymouth PL4 8AA, Devon, England
[7] Univ Santiago Chile, Fac Quim & Biol, Lab Marine Biotechnol, Santiago 9170020, Chile
[8] Univ Playa Ancha, HUB AMBIENTAL UPLA, Valparaiso 2340000, Chile
关键词
in vivo chlorophyll a florescence; physiology; mitogen activated protein kinases; Ulva compressa; metal accumulation; ACTIVATED PROTEIN-KINASE; ECTOCARPUS-SILICULOSUS; INTERTIDAL MACROALGAE; ENVIRONMENTAL-STRESS; DUNALIELLA-VIRIDIS; CROSS-TALK; RESPONSES; PHOSPHORYLATION; CALCIUM; ANTIOXIDANT;
D O I
10.3390/ijms20184547
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There is currently no information regarding the role that whole mitogen activated protein kinase (MAPK) pathways play in counteracting environmental stress in photosynthetic organisms. To address this gap, we exposed Ulva compressa to chronic levels of copper (10 mu M) specific inhibitors of Extracellular Signal Regulated Kinases (ERK), c-Jun N-terminal Kinases (JNK), and Cytokinin Specific Binding Protein (p38) MAPKs alone or in combination. Intracellular copper accumulation and photosynthetic activity (in vivo chlorophyll a fluorescence) were measured after 6 h, 24 h, 48 h, and 6 days of exposure. By day 6, when one (except JNK) or more of the MAPK pathways were inhibited under copper stress, there was a decrease in copper accumulation compared with algae exposed to copper alone. When at least two MAPKs were blocked, there was a decrease in photosynthetic activity expressed in lower productivity (ETRmax), efficiency (alpha(ETR)), and saturation of irradiance (EkETR), accompanied by higher non-photochemical quenching (NPQ(max)), compared to both the control and copper-only treatments. In terms of accumulation, once the MAPK pathways were partially or completely blocked under copper, there was crosstalk between these and other signaling mechanisms to enhance metal extrusion/exclusion from cells. Crosstalk occurred among MAPK pathways to maintain photosynthesis homeostasis, demonstrating the importance of the signaling pathways for physiological performance. This study is complemented by a parallel/complementary article Rodriguez-Rojas et al. on the role of MAPKs in copper-detoxification.
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页数:16
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共 63 条
  • [1] Metabolic switching: synergistic induction of carotenogenesis in the aerial microalga, Vischeria helvetica, under environmental stress conditions by inhibitors of fatty acid biosynthesis
    Aburai, Nobuhiro
    Abe, Katsuya
    [J]. BIOTECHNOLOGY LETTERS, 2015, 37 (05) : 1073 - 1080
  • [2] Anderson MJ., 2008, PERMANOVA+ for PRIMER: Guide to Software and Statistical Methods, P218
  • [3] Ecophysiological and metabolic responses to interactive exposure to nutrients and copper excess in the brown macroalga Cystoseira tamariscifolia
    Celis-Pla, Paula S. M.
    Brown, Murray T.
    Santillan-Sarmiento, Alex
    Korbee, Nathalie
    Saez, Claudio A.
    Figueroa, Felix L.
    [J]. MARINE POLLUTION BULLETIN, 2018, 128 : 214 - 222
  • [4] Seasonal biochemical and photophysiological responses in the intertidal macroalga Cystoseira tamariscifolia (Ochrophyta)
    Celis-Pla, Paula S. M.
    Bouzon, Zenilda L.
    Hall-Spencer, Jason M.
    Schmidt, Eder C.
    Korbee, Nathalie
    Figueroa, Felix L.
    [J]. MARINE ENVIRONMENTAL RESEARCH, 2016, 115 : 89 - 97
  • [5] Seasonal photoacclimation patterns in the intertidal macroalga Cystoseira tamariscifolia (Ochrophyta)
    Celis-Pla, Paula S. M.
    Korbee, Nathalie
    Gomez-Garreta, Amelia
    Figueroa, Felix L.
    [J]. SCIENTIA MARINA, 2014, 78 (03) : 377 - 388
  • [6] Inhibition of JNK and ERK Pathways by SP600125-and U0126-Enhanced Osteogenic Differentiation of Bone Marrow Stromal Cells
    Doan, Thi Kim Phuong
    Park, Kyung Soon
    Kim, Hyung Keun
    Park, Due Sung
    Kim, Ji Hyun
    Yoon, Taek Rim
    [J]. TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2012, 9 (06) : 283 - 294
  • [7] A MODEL FOR THE RELATIONSHIP BETWEEN LIGHT-INTENSITY AND THE RATE OF PHOTOSYNTHESIS IN PHYTOPLANKTON
    EILERS, PHC
    PEETERS, JCH
    [J]. ECOLOGICAL MODELLING, 1988, 42 (3-4) : 199 - 215
  • [8] Overview of eutrophication indicators to assess environmental status within the European Marine Strategy Framework Directive
    Ferreira, Joao G.
    Andersen, Jesper H.
    Borja, Angel
    Bricker, Suzanne B.
    Camp, Jordi
    da Silva, Margarida Cardoso
    Garces, Esther
    Heiskanen, Anna-Stiina
    Humborg, Christoph
    Ignatiades, Lydia
    Lancelot, Christiane
    Menesguen, Alain
    Tett, Paul
    Hoepffner, Nicolas
    Claussen, Ulrich
    [J]. ESTUARINE COASTAL AND SHELF SCIENCE, 2011, 93 (02) : 117 - 131
  • [9] Relations between electron transport rates determined by pulse amplitude modulated chlorophyll fluorescence and oxygen evolution in macroalgae under different light conditions
    Figueroa, FL
    Conde-Alvarez, R
    Gómez, I
    [J]. PHOTOSYNTHESIS RESEARCH, 2003, 75 (03) : 259 - 275
  • [10] Insight on signal transduction pathways involved in phagocytosis in the colonial ascidian Botryllus schlosseri
    Franchi, Nicola
    Schiavon, Filippo
    Betti, Michele
    Canesi, Laura
    Ballarin, Loriano
    [J]. JOURNAL OF INVERTEBRATE PATHOLOGY, 2013, 112 (03) : 260 - 266