Effect of different level of monensin supplemented with cold process urea molasses mineral block on in vitro rumen fermentation at different days of adaptation with monensin

Effect of period of adaptation and levels of monensin were studied for microbial fermentation/digestibility to find out the optimum period of adaptation of monensin in rumen and suitable level of monensin in wheat straw+concentrate and wheat straw+ UMMB diet. The mean digestibility of dry matter was decreased upto T-3 treatment (49.17%), however, digestibility of DM was affected upto period (P-2). NDF digestibility was affected due to treatment under P1 and P2 (p<0.05). Average digestibility of ADF was increased to 53.33% at T-3 level of monensin and P4 days of adaptation. TVFA (mmole/100 ml) were decreased from 9.49 in T-1 to 7,70 in T-7. Periods were not effectives except P2 (14 days of adaptation). Similarly, total gas was decrease with the increase of monensin levels in diet. Although acetate percentage in TVFA was not affected either due to level of monensin or period of adaptation but propionate was increased due to increase in monensin at 21 days of adaptation (P-3). Butyrate (%) was decreased significantly in T-2 to T-6 as compared to T-I group. Total gas was significantly (p<0.01) higher in group T-I (control) and it reduced significantly in T-5, however, differences in gas production between group T-3, T-5 and T-7 at P-1 was not significant. Methane production was reduced on P-3 and P-4 level of adaptation due to treatment. The overall result indicated that 2 1 days of adaptation with monensin was sufficient to mask the inhibiting effect of monensin to cell wall digestibility and 35 ppm monensin is optimum to reduce methane production and increase propionate productions.