Bioallethrin enhances generation of ROS, damages DNA, impairs the redox system and causes mitochondrial dysfunction in human lymphocytes

被引:13
作者
Arif, Amin [1 ]
Quds, Ruhul [1 ]
Mahmood, Riaz [1 ]
机构
[1] Aligarh Muslim Univ, Fac Life Sci, Dept Biochem, Aligarh 202002, Uttar Pradesh, India
关键词
OXIDATIVE STRESS; CATALASE INACTIVATION; ALPHA-CYPERMETHRIN; ALLETHRIN TOXICITY; LAMBDA-CYHALOTHRIN; CELL-DEATH; APOPTOSIS; GLUTATHIONE; EXPOSURE; OXYGEN;
D O I
10.1038/s41598-021-87799-3
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bioallethrin is a synthetic pesticide that is widely used to control insect pests. The wide use of bioallethrin has resulted in inevitable human exposure. In this study we report the effect of different concentrations of bioallethrin (10 to 200 mu M, 2 h at 37 degrees C) on human lymphocytes under in vitro conditions. Bioallethrin treatment resulted in loss of cell viability (>30% at 200 mu M bioallethrin). Oxidative stress markers like lipid peroxidation and protein oxidation were significantly increased accompanied by lower ratio of reduced to oxidized glutathione. Enhanced ROS generation was observed through fluorescence spectroscopy and microscopy. Bioallethrin-induced oxidative stress also compromised the antioxidant defence as it reduced antioxidant capacity of cells and inhibited major antioxidant enzymes. Biomolecular modifications and systemic toxicity by bioallethrin resulted in plasma membrane damage with mitochondrial depolarization. Comet assay showed nuclear DNA fragmentation and strand scission with significant increase in tail length and olive tail moment. Apoptosis and necrosis of cells was confirmed through acridine orange/ethidium bromide dual staining and visualization under fluorescence microscope. Thus, bioallethrin causes oxidative damage and compromises the antioxidant system leading to DNA damage, cellular and organelle toxicity, resulting in apoptosis and necrosis of human lymphocytes.
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页数:13
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