TET2 Regulates LncRNA-ANRIL Expression and Inhibits the Growth of Human Gastric Cancer Cells

被引：56
作者：
Deng, Wei [1 ,2 ]
Wang, Jin [1 ,2 ]
Zhang, Jun [1 ,2 ]
Cai, Jun [1 ,2 ]
Bai, Zhigang [1 ,2 ]
Zhang, Zhongtao [1 ,2 ]
机构：
[1] Capital Med Univ, Beijing Friendship Hosp, Dept Gen Surg, 95 Yongan Rd, Beijing 100050, Peoples R China
[2] Natl Clin Res Ctr Digest Dis, Beijing, Peoples R China
来源：
IUBMB LIFE | 2016年 / 68卷 / 05期
关键词：
TET2; gastric cancer; lncRNA-ANRIL; apoptosis; NONCODING RNA ANRIL; MYELOID MALIGNANCIES; SIGNALING PATHWAY; LONG; COMMON; APOPTOSIS; MUTATIONS;
D O I：
10.1002/iub.1490
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Ten-eleven translocation (TET) family enzymes convert 5-methylcytosine to 5-hydroxymethylcytosine in DNA. However, the role of TET enzymes in human gastric cancer remains unknown. Here, we show that TET2 mRNA and protein levels are downregulated in human gastric cancer tissues when compared with normal gastric mucosa. Low expression of TET2 predicts poor overall and disease-free survival. Furthermore, we demonstrate that TET2 inhibits the proliferation and colony formation of human gastric cancer cells by using loss-of-function and gain-of-function strategies. Overexpression of TET2 induces apoptosis in human gastric cancer cells. The mechanism study shows that TET2 binds to the promoter region of the oncogenic long noncoding RNA (lncRNA-ANRIL) and regulates the expression of ANRIL and its downstream genes (INK4a, INK4b, and ARF). Finally, we demonstrate that ANRIL knockdown blocks the effects of TET2 on gastric cancer cell proliferation and colony formation. (C) 2016 IUBMB Life.
引用
收藏
页码：355 / 364
页数：10
相关论文
共 22 条
  • [1] Genetic characterization of TET1, TET2, and TET3 alterations in myeloid malignancies
    Abdel-Wahab, Omar
    Mullally, Ann
    Hedvat, Cyrus
    Garcia-Manero, Guillermo
    Patel, Jay
    Wadleigh, Martha
    Malinge, Sebastien
    Yao, JinJuan
    Kilpivaara, Outi
    Bhat, Rukhmi
    Huberman, Kety
    Thomas, Sabrena
    Dolgalev, Igor
    Heguy, Adriana
    Paietta, Elisabeth
    Le Beau, Michelle M.
    Beran, Miloslav
    Tallman, Martin S.
    Ebert, Benjamin L.
    Kantarjian, Hagop M.
    Stone, Richard M.
    Gilliland, D. Gary
    Crispino, John D.
    Levine, Ross L.
    [J]. BLOOD, 2009, 114 (01) : 144 - 147
  • [2] ANRIL inhibits p15INK4b through the TGFβ1 signaling pathway in human esophageal squamous cell carcinoma
    Chen, Deyu
    Zhang, Zhaoyue
    Mao, Chaoming
    Zhou, Yuepeng
    Yu, Lichao
    Yin, Yue
    Wu, Shi
    Mou, Xiao
    Zhu, Yan
    [J]. CELLULAR IMMUNOLOGY, 2014, 289 (1-2) : 91 - 96
  • [3] TET2 promotes histone O-GlcNAcylation during gene transcription
    Chen, Qiang
    Chen, Yibin
    Bian, Chunjing
    Fujiki, Ryoji
    Yu, Xiaochun
    [J]. NATURE, 2013, 493 (7433) : 561 - +
  • [4] Mutation in TET2 in Myeloid Cancers
    Delhommeau, Francois
    Dupont, Sabrina
    Della Valle, Veronique
    James, Chloe
    Trannoy, Severine
    Masse, Aline
    Kosmider, Olivier
    Le Couedic, Jean-Pierre
    Robert, Fabienne
    Alberdi, Antonio
    Lecluse, Yann
    Plo, Isabelle
    Dreyfus, Francois J.
    Marzac, Christophe
    Casadevall, Nicole
    Lacombe, Catherine
    Romana, Serge P.
    Dessen, Philippe
    Soulier, Jean
    Viguie, Franck
    Fontenay, Michaela
    Vainchenker, William
    Bernard, Olivier A.
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2009, 360 (22) : 2289 - 2301
  • [5] Tumor markers in colorectal cancer, gastric cancer and gastrointestinal stromal cancers: European group on tumor markers 2014 guidelines update
    Duffy, M. J.
    Lamerz, R.
    Haglund, C.
    Nicolini, A.
    Kalousova, M.
    Holubec, L.
    Sturgeon, C.
    [J]. INTERNATIONAL JOURNAL OF CANCER, 2014, 134 (11) : 2513 - 2522
  • [6] Decreased expression of ten-eleven translocation 1 protein is associated with some clinicopathological features in gastric cancer
    Frycz, Bartosz Adam
    Murawa, Dawid
    Borejsza-Wysocki, Maciej
    Marciniak, Ryszard
    Murawa, Pawel
    Drews, Michal
    Kolodziejczak, Anna
    Tomela, Katarzyna
    Jagodzinski, Pawel Piotr
    [J]. BIOMEDICINE & PHARMACOTHERAPY, 2014, 68 (02) : 209 - 212
  • [7] TET1 Exerts Its Tumor Suppressor Function by Interacting with p53-EZH2 Pathway in Gastric Cancer
    Fu, Hua-Lin
    Ma, Yue
    Lu, Lun-Gen
    Hou, Peng
    Li, Bao-Jie
    Jin, Wei-Lin
    Cui, Da-Xiang
    [J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2014, 10 (07) : 1217 - 1230
  • [8] Huang M., 2015, J HEMATOL ONCOL, V8, P1
  • [9] Jia Y., 2014, MATH PROBL ENG, V2014, P1, DOI DOI 10.1371/J0URNAL.P0NE.0097999
  • [10] Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2
    Ko, Myunggon
    Huang, Yun
    Jankowska, Anna M.
    Pape, Utz J.
    Tahiliani, Mamta
    Bandukwala, Hozefa S.
    An, Jungeun
    Lamperti, Edward D.
    Koh, Kian Peng
    Ganetzky, Rebecca
    Liu, X. Shirley
    Aravind, L.
    Agarwal, Suneet
    Maciejewski, Jaroslaw P.
    Rao, Anjana
    [J]. NATURE, 2010, 468 (7325) : 839 - 843
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