PEG-modified glucose oxidase immobilized on a PVA cryogel membrane for amperometric biosensor applications

被引：50
作者：
Doretti, L
Ferrara, D
Gattolin, P
Lora, S
Schiavon, F
Veronese, FM
机构：
[1] CNR, Uffcio Sicurezza & Prevenz, I-35127 Padua, Italy
[2] CNR, Ist Fotochim & Radiaz Alta Energia, Sez Legnaro, I-35020 Padua, Italy
[3] Univ Padua, Dipartimento Sci Farmaceut, I-35131 Padua, Italy
来源：
TALANTA | 1998年 / 45卷 / 05期
关键词：
biosensors; glucose oxidase; PEG-modified glucose oxidase; PVA cryogel;
D O I：
10.1016/S0039-9140(97)00191-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Poly(ethylene glycol)-modified glucose oxidase was immobilized in a poly(vinyl alcohol) cryogel membrane, obtained by a freezing-thawing cyclic process, to obtain a suitable amperometric glucose sensor, The covalent linkage between PEG and GOD molecule improved the physical immobilization of enzyme in the polymeric matrix, by decreasing its loss in time. Sensor behaviour was evaluated electrochemically with a hydrogen peroxide electrode, The glucose content in standard solutions was determined and linear calibration curves in the 5 x 10(-5)-5 x 10(-3) mol l(-1) range were obtained. The kinetic parameters in the immobilized system were evaluated and analytical characteristics of sensor, including stability and influence of pH and temperature, were determined. (C) 1998 Elsevier Science B.V.
引用
收藏
页码：891 / 898
页数：8
相关论文
共 20 条
[1]   ACTIVE-SITE PROTECTION OF PROTEOLYTIC-ENZYMES BY POLY(ETHYLENE GLYCOL) SURFACE MODIFICATION [J].
CALICETI, P ;
SCHIAVON, O ;
SARTORE, L ;
MONFARDINI, C ;
VERONESE, FM .
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, 1993, 8 (01) :41-50
[2]   Cyro-hydrogel for the construction of a tyrosinase-based biosensor [J].
Deng, Q ;
Guo, YZ ;
Dong, SJ .
ANALYTICA CHIMICA ACTA, 1996, 319 (1-2) :71-77
[3]   Amperometric biosensor with physically immobilized glucose oxidase on a PVA cryogel membrane [J].
Doretti, L ;
Ferrara, D ;
Gattolin, P ;
Lora, S .
TALANTA, 1997, 44 (05) :859-866
[4]   IMMOBILIZATION OF GLUCOSE-OXIDASE IN A POLY [VINYL ALCOHOL] MATRIX ON PLATINIZED GRAPHITE-ELECTRODES BY GAMMA-IRRADIATION [J].
GALIATSATOS, C ;
IKARIYAMA, Y ;
MARK, JE ;
HEINEMAN, WR .
BIOSENSORS & BIOELECTRONICS, 1990, 5 (01) :47-61
[5]  
GUIBAULT GG, 1973, ANAL CHIM ACTA, V64, P439
[6]   ROLE OF CARBONYL SITES IN THE ELECTROCHEMICAL RESPONSE OF 2,6-DIAMINOPURIN-8-OL [J].
BODALBHAI, L ;
BRAJTERTOTH, A .
ANALYTICA CHIMICA ACTA, 1991, 243 (01) :33-42
[7]   IMMOBILIZATION OF LACTATE OXIDASE IN A POLY(VINYL ALCOHOL) MATRIX ON PLATINIZED GRAPHITE-ELECTRODES BY CHEMICAL CROSS-LINKING WITH ISOCYANATE [J].
HAJIZADEH, K ;
HALSALL, HB ;
HEINEMAN, WR .
TALANTA, 1991, 38 (01) :37-47
[8]   IMMOBILIZATION OF ENZYME INTO POLYVINYL-ALCOHOL) MEMBRANE [J].
IMAI, K ;
SHIOMI, T ;
UCHIDA, K ;
MIYA, M .
BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (11) :1721-1726
[9]   Electromagnetic field stimulation of biosynthesis: Changes in c-myc transcript levels during continuous and intermittent exposures [J].
Lin, H ;
Blank, M ;
Jin, M ;
Goodman, R .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1996, 39 (02) :215-220
[10]   APPLICATION OF PHOTOCROSSLINKABLE POLYVINYL-ALCOHOL) TO ENZYME MEMBRANES FOR BIOSENSORS [J].
MATSUMOTO, K ;
MIZUGUCHI, H ;
ICHIMURA, K .
KOBUNSHI RONBUNSHU, 1984, 41 (04) :221-228
12