Molecular Biomarkers for Weight Control in Obese Individuals Subjected to a Multiphase Dietary Intervention

被引:29
作者
Bolton, Jennifer [1 ,2 ]
Montastier, Emilie [1 ,2 ,3 ,4 ,5 ]
Carayol, Jerome [6 ]
Bonnel, Sophie [1 ,2 ]
Mir, Lucile [1 ,2 ]
Marques, Marie-Adeline [1 ,2 ]
Astrup, Arne [7 ]
Saris, Wim [8 ]
Iacovoni, Jason [1 ,2 ]
Villa-Vialaneix, Nathalie [9 ]
Valsesia, Armand [6 ]
Langin, Dominique [1 ,2 ,3 ,4 ,5 ]
Viguerie, Nathalie [1 ,2 ]
机构
[1] INSERM, Obes Res Lab, Inst Metab & Cardiovasc Dis, F-31432 Toulouse, France
[2] Paul Sabatier Univ, Univ Toulouse, F-31400 Toulouse, France
[3] Toulouse Univ Hosp, Dept Endocrinol, F-31400 Toulouse, France
[4] Toulouse Univ Hosp, Dept Metab, F-31400 Toulouse, France
[5] Toulouse Univ Hosp, Dept Nutr, F-31400 Toulouse, France
[6] Nestle Inst Hlth Sci SA, CH-1015 Lausanne, Switzerland
[7] Univ Copenhagen, Dept Nutr Exercise & Sports, Fac Sci, DK-2200 Copenhagen, Denmark
[8] Maastricht Univ, Med Ctr, NUTRIM Sch Nutr & Translat Res Metab, Dept Human Biol, NL-6229 Maastricht, Netherlands
[9] Univ Toulouse, Unite Math & Informat Appl Toulouse, F-31326 Castanet Tolosan, France
关键词
TISSUE GENE-EXPRESSION; ADIPOSE-TISSUE; SKELETAL-MUSCLE; TGF-BETA; ADIPOCYTE; RESTRICTION; FAT; MACROPHAGES; FOLLISTATIN; GLUCOSE;
D O I
10.1210/jc.2016-3997
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Context: Although calorie restriction has proven beneficial for weight loss, long-term weight control is variable between individuals. Objective: To identify biomarkers of successful weight control during a dietary intervention (DI). Design, Setting, and Participants: Adipose tissue (AT) transcriptomes were compared between 21 obese individuals who either maintained weight loss or regained weight during the DI. Results were validated on 310 individuals from the same study using quantitative reverse transcription polymerase chain reaction and protein levels of potential circulating biomarkers measured by enzyme-linked immunosorbent assay. Intervention: Individuals underwent 8 weeks of low-calorie diet, then 6 months of ad libitum diet. Outcome Measure: Weight changes at the end of the DI. Results: We evaluated six genes that had altered expression during DI, encode secreted proteins, and have not previously been implicated in weight control (EGFL6, FSTL3, CRYAB, TNMD, SPARC, IGFBP3), as well as genes for which baseline expression differed between those with good and poor weight control (ASPN, USP53). Changes in plasma concentrations of EGFL6, FSTL3, and CRYAB mirrored AT messenger RNA expression; all decreased during DI in individuals with good weight control. ASPN and USP53 had higher baseline expression in individuals who went on to have good weight control. Expression quantitative trait loci analysis found polymorphisms associated with expression levels of USP53 in AT. A regulatory network was identified in which transforming growth factor beta 1 (TGF-beta 1) was responsible for downregulation of certain genes during DI in good controllers. Interestingly, ASPN is a TGF-beta 1 inhibitor. Conclusions: We found circulating biomarkers associated with weight control that could influence weight management strategies and genes that may be prognostic for successful weight control.
引用
收藏
页码:2751 / 2761
页数:11
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