Virus-triggered Ubiquitination of TRAF3/6 by cIAP1/2 Is Essential for Induction of Interferon-β (IFN-β) and Cellular Antiviral Response

被引：115
作者：
Mao, Ai-Ping [1 ]
Li, Shu [1 ]
Zhong, Bo [1 ]
Li, Ying [1 ]
Yan, Jie [1 ]
Li, Qi [1 ]
Teng, Chengwen [1 ]
Shu, Hong-Bing [1 ]
机构：
[1] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 2010年 / 285卷 / 13期
基金：
中国国家自然科学基金;
关键词：
NF-KAPPA-B; NEGATIVE REGULATION; SIGNALING COMPLEX; ADAPTER PROTEIN; ACTIVATION PATHWAYS; I INTERFERON; LIGASE; INNATE; KINASE; TRANSDUCTION;
D O I：
10.1074/jbc.M109.071043
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Viral infection causes activation of transcription factors NF-kappa B and IRF3, which collaborate to induce type I interferons (IFNs) and cellular antiviral response. Here we show that knockdown of the E3 ubiquitin ligases cIAP1 and cIAP2 markedly inhibited virus-triggered activation of IRF3 and NF-kappa B as well as IFN-beta induction. Knockdown of cIAP1 and cIAP2 also inhibited cytoplasmic dsRNA-triggered cellular antiviral response. Endogenous coimmunoprecipitation experiments indicated that viral infection caused recruitment of cIAP1 and cIAP2 to TRAF3, TRAF6, and VISA. Furthermore, we demonstrated that cIAP1- and cIAP2-mediated virus-triggered ubiquitination of TRAF3 and TRAF6. These findings suggest that virus-triggered ubiquitination of TRAF3 and TRAF6 by cIAP1 and cIAP2 is essential for type I IFN induction and cellular antiviral response.
引用
收藏
页码：9470 / 9476
页数：7
相关论文
共 36 条
  • [1] Negative regulation of the RIG-I signaling by the ubiquitin ligase RNF125
    Arimoto, Kei-ichiro
    Takahashi, Hitoshi
    Hishiki, Takayuki
    Konishi, Hicleyuki
    Fujita, Takashi
    Shimotohno, Kunitada
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (18) : 7500 - 7505
  • [2] Cellular Inhibitors of Apoptosis cIAP1 and cIAP2 Are Required for Innate Immunity Signaling by the Pattern Recognition Receptors NOD1 and NOD2
    Bertrand, Mathieu J. M.
    Doiron, Karine
    Labbe, Katherine
    Korneluk, Robert G.
    Barker, Philip A.
    Saleh, Maya
    [J]. IMMUNITY, 2009, 30 (06) : 789 - 801
  • [3] Negative regulation of MDA5-but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase
    Diao, Feici
    Li, Shu
    Tian, Yang
    Zhang, Min
    Xu, Liang-Guo
    Zhang, Yan
    Wang, Rui-Peng
    Chen, Danying
    Zhai, Zhonghe
    Zhong, Bo
    Tien, Po
    Shu, Hong-Bing
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (28) : 11706 - 11711
  • [4] Type IIFN modulates innate and specific antiviral immunity
    Durbin, JE
    Fernandez-Sesma, A
    Lee, CK
    Rao, TD
    Frey, AB
    Moran, TM
    Vukmanovic, S
    García-Sastre, A
    Levy, DE
    [J]. JOURNAL OF IMMUNOLOGY, 2000, 164 (08) : 4220 - 4228
  • [5] TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity
    Gack, Michaela U.
    Shin, Young C.
    Joo, Chul-Hyun
    Urano, Tomohiko
    Liang, Chengyu
    Sun, Lijun
    Takeuchi, Osamu
    Akira, Shizuo
    Chen, Zhijian
    Inoue, Satoshi
    Jung, Jae U.
    [J]. NATURE, 2007, 446 (7138) : 916 - U2
  • [6] REUL Is a Novel E3 Ubiquitin Ligase and Stimulator of Retinoic-Acid-Inducible Gene-I
    Gao, Dong
    Yang, Yong-Kang
    Wang, Rui-Peng
    Zhou, Xiang
    Diao, Fei-Ci
    Li, Min-Dian
    Zhai, Zhong-He
    Jiang, Zheng-Fan
    Chen, Dan-Ying
    [J]. PLOS ONE, 2009, 4 (06):
  • [7] Specificity in Toll-like receptor signalling through distinct effector functions of TRAF3 and TRAF6
    Häcker, H
    Redecke, V
    Blagoev, B
    Kratchmarova, I
    Hsu, LC
    Wang, GG
    Kamps, MP
    Raz, E
    Wagner, H
    Häcker, G
    Mann, M
    Karin, M
    [J]. NATURE, 2006, 439 (7073) : 204 - 207
  • [8] Type I inteferon gene induction by the interferon regulatory factor family of transcription factors
    Honda, Kenya
    Takaoka, Akinori
    Taniguchi, Tadatsugu
    [J]. IMMUNITY, 2006, 25 (03) : 349 - 360
  • [9] SIKE is an IKKε/TBK1-associated suppressor of TLR3-and virus-triggered IRF-3 activation pathways
    Huang, J
    Liu, T
    Xu, LG
    Chen, DY
    Zhai, ZH
    Shu, HB
    [J]. EMBO JOURNAL, 2005, 24 (23) : 4018 - 4028
  • [10] STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling
    Ishikawa, Hiroki
    Barber, Glen N.
    [J]. NATURE, 2008, 455 (7213) : 674 - U74
1234