Effect of SIRT1 on Apoptosis of Bovine Preadipocytes

被引：3
作者：
Liu Xiao-Mu [1 ,2 ]
Song En-Liang [1 ,2 ]
Liu Gui-Fen [1 ,2 ]
Wu Gong-Ying [3 ]
Tan Xiu-Wen [1 ,2 ]
Liu Zhen-Shan [1 ,2 ]
Wan Fa-Chun [1 ,2 ]
机构：
[1] Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China
[2] Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China
[3] Shandong Vocat Anim Sci & Vet Coll, Weifang 261061, Peoples R China
来源：
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2010年 / 37卷 / 03期
关键词：
SIRT1; nicotinamide; bovine; preadipocytes; apoptosis; GENE-EXPRESSION; ADIPOSE-TISSUE; DIFFERENTIATION; NICOTINAMIDE; MUSCLE; PROLIFERATION; ADIPOCYTES; INHIBITION; REGULATOR; FOXO;
D O I：
10.3724/SP.J.1206.2009.00583
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Sirtuin type 1 (SIRT1), a novel regulatory factor of adipocyte and myocyte, interacts with target the forkhead box O family 1 (FoxO1) to modulate cell proliferation and differentiation, aging, apotosis and metabolism. The effect of nicotinamide (NAM, inhibitor of SIRT1) on the apoptosis of bovine preadipocytes was investigated with Acridine Orange (AO) staining, the flow cytometry detection and quantitative real-time PCR (qPCR). The results showed that NAM inhibited the differentiation of bovine subcutaneous preadipocytes (BSP) and bovine intramuscular preadipocytes (BIP), and their apoptosis rates were higher than control. SIRT1 influenced the apoptosis of bovine preadipocytes by regulating FoxO1 and adipocyte marker genes. The different mechanisms of SIRT1 and its related genes exist in BSP and BIP.
引用
收藏
页码：297 / 303
页数：7
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