Gold nanorods for fluorescence lifetime imaging in biology

被引：53

作者：

Zhang, Yinan ^{[1
]}

Yu, Jun ^{[2
]}

Birch, David J. S. ^{[1
]}

Chen, Yu ^{[1
]}

机构：

[1] Univ Strathclyde, SUPA, Dept Phys, Ctr Mol Nanometrol,Photophys Grp, Glasgow G4 0NG, Lanark, Scotland

[2] Univ Strathclyde, Royal Coll, Strathclyde Inst Pharm & Biomed Sci, Glasgow G1 1XW, Lanark, Scotland

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 02期

基金：

英国工程与自然科学研究理事会;

关键词：

gold nanorods; two-photon luminescence; fluorescence lifetime imaging microscopy; CONTRAST AGENTS; CELLS; LUMINESCENCE; NANOPARTICLES; ENHANCEMENT; MICROSCOPY; ABSORPTION; EMISSION; PLASMON; METAL;

D O I：

10.1117/1.3366646

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3366646]

引用

页数：3

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