Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma β-cells (INS-1)

被引：19

作者：

Bazwinsky-Wutschke, Ivonne ^{[1
]}

Wolgast, Sabine ^{[2
]}

Muehlbauer, Eckhard ^{[2
]}

Peschke, Elmar ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Inst Anat & Cell Biol, D-06097 Halle, Germany

[2] Saxon Acad Sci Leipzig, D-04107 Leipzig, Germany

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2010年 / 134卷 / 02期

关键词：

Secretagogin; Calmodulin; Calbindin-D28k; Calreticulin; Pancreatic islet; INS-1; Immunohistochemistry; Molecular biology; SUPERIOR OLIVARY COMPLEX; ENDOPLASMIC-RETICULUM; GENE-EXPRESSION; GLUCOSE; ISLETS; CALMODULIN; CA2+; GK; CALRETICULIN; CALBINDIN-D-28K;

D O I：

10.1007/s00418-010-0721-y

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The present study dealt with the localization of different calcium-binding proteins (CaBPs) in the pancreatic tissue of non-diabetic and diabetic rats and in rat insulinoma beta-cells (INS-1). Transcripts of CaBPs displayed different expression levels in rat pancreatic tissue and INS-1 cells. Immunohistochemistry demonstrated that three of these proteins, calmodulin, calreticulin and calbindin-D28k, were located predominantly in the pancreatic islets (in both alpha- and beta-cells) of rats, showing weaker labeling of exocrine tissue. Secretagogin was exclusively found within islets. All CaBPs were also immunohistochemically detected in INS-1 cells. Immunohistochemical analysis demonstrates differences in CaBP distributions when comparing the pancreatic tissues of diabetic Goto-Kakizaki rats and non-diabetic Wistar rats. Pancreatic tissue in type 2 diabetic Goto-Kakizaki rats showed significantly higher transcript levels of all CaBPs compared to those in Wistar rats. These results indicate that alterations of CaBPs in pancreatic islets are associated with metabolic disturbances related to type 2 diabetes.

引用

页码：115 / 127

页数：13

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