Determination of Cry1Ac copy number in transgenic pigeonpea plants using quantitative real time PCR

被引：7

作者：

Jain, Meenakshi ^{[1
]}

Khatodia, Surender ^{[1
]}

Kharb, Pushpa ^{[1
]}

Batra, Parveen ^{[1
]}

Chowdhury, Vijay K. ^{[1
]}

机构：

[1] Chaudhary Charan Singh Haryana Agr Univ, Dept Mol Biol & Biotechnol, Hisar 125004, Haryana, India

来源：

LEGUME RESEARCH | 2017年 / 40卷 / 04期

关键词：

Copy number; Cry1Ac; Pigeonpea transformation; Real-time PCR; Syber green; GENE; ASSAY; COPIES;

D O I：

10.18805/lr.v0iOF.11300

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Copy number of CrylAc in transgenic pigeonpea plants was determined by quantitative real time PCR using Syber Green as fluorescence indicator. Gene specific primers designed to amplify relatively long amplicons (400 - 600 bp), for CrylAc was used to increase specificity and sensitivity of Real time PCR. Estimated copy number in transgenic lines using realtime quantitative PCR and southern hybridization was correlated and found to be same Le. single copy number. This study shows effectivness of real-time PCR method for estimating the transgene copy number in transgenic pigeonpea plants by a simple, accurate and cost effective manner.

引用

页码：643 / 648

页数：6

共 22 条

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