Effect of Gold Nanoparticle Size on Their Properties as Contrast Agents for Computed Tomography

被引:172
作者
Dong, Yuxi C. [1 ,2 ]
Hajfathalian, Maryam [1 ]
Maidment, Portia S. N. [1 ]
Hsu, Jessica C. [1 ,2 ]
Naha, Pratap C. [1 ]
Si-Mohamed, Salim [4 ,5 ]
Breuilly, Marine [5 ]
Kim, Johoon [1 ,2 ]
Chhour, Peter [1 ]
Douek, Philippe [4 ,5 ]
Litt, Harold I. [1 ,3 ]
Cormode, David P. [1 ,2 ,3 ]
机构
[1] Univ Penn, Dept Radiol, 3400 Spruce St,1 Silverstein, Philadelphia, PA 19104 USA
[2] Univ Penn, Dept Bioengn, 3400 Spruce St,1 Silverstein, Philadelphia, PA 19104 USA
[3] Univ Penn, Dept Med, 3400 Spruce St,1 Silverstein, Philadelphia, PA 19104 USA
[4] Hop Cardiovasc & Pneumol Louis Pradel, Dept Radiol, Lyon, France
[5] Univ Lyon1 Claude Bernard, CREATIS, CNRS, UMR 5220,Inserm,U1044, Lyon, France
基金
欧盟地平线“2020”;
关键词
X-RAY ATTENUATION; IN-VIVO BIODISTRIBUTION; EXPERIMENTAL FEASIBILITY; IMAGING PROBE; CT; STABILITY; LIPOSOMES; CANCER; SILVER;
D O I
10.1038/s41598-019-50332-8
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Computed tomography (CT) is one of the most commonly used clinical imaging modalities. There have recently been many reports of novel contrast agents for CT imaging. In particular, the development of gold nanoparticles (AuNP) as CT contrast agents is a topic of intense interest. AuNP have favorable characteristics for this application such as high payloads of contrast generating material, strong X-ray attenuation, excellent biocompatibility, tailorable surface chemistry, and tunable sizes and shapes. However, there have been conflicting reports on the role of AuNP size on their contrast generation for CT. We therefore sought to extensively investigate the AuNP size-CT contrast relationship. In order to do this, we synthesized AuNP with sizes ranging from 4 to 152 nm and capped them with 5 kDa m-PEG. The contrast generation of AuNP of different sizes was investigated with three clinical CT, a spectral photon counting CT (SPCCT) and two micro CT systems. X-ray attenuation was quantified as attenuation rate in Hounsfield units per unit concentration (HU/mM). No statistically significant difference in CT contrast generation was found among different AuNP sizes via phantom imaging with any of the systems tested. Furthermore, in vivo imaging was performed in mice to provide insight into the effect of AuNP size on animal biodistribution at CT dose levels, which has not previously been explored. Both in vivo imaging and ex vivo analysis with inductively coupled plasma optical emission spectroscopy (ICP-OES) indicated that AuNP that are 15 nm or smaller have long blood circulation times, while larger AuNP accumulated in the liver and spleen more rapidly. Therefore, while we observed no AuNP size effect on CT contrast generation, there is a significant effect of size on AuNP diagnostic utility.
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页数:13
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