Expression of GnRH receptor in mouse and rat testicular germ cells

被引:35
作者
Bull, P
Morales, P
Huyser, C
Socías, T
Castellón, EA
机构
[1] Univ Antofagasta, Fac Hlth Sci, Reprod Biol Unit, Antofagasta, Chile
[2] Pontificia Univ Catolica Chile, Dept Microbiol & Mol Genet, Santiago, Chile
[3] Univ Pretoria, Dept Obstet & Gynaecol, Reprod Biol Lab, ZA-0002 Pretoria, South Africa
[4] Univ Chile, ICBM, Santiago, Chile
关键词
GnRH; GnRH receptors; spermatozoa; spermatogenic cells; testis;
D O I
10.1093/molehr/6.7.582
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The expression of gonadotrophin-releasing hormone receptor (GnRH-R) in germinal cells of mouse testis, whole testes, pituitary glands, and mouse ovaries was determined by means of Northern hybridization using a mouse GnRH-R [P-32]-labelled cDNA probe. Also, the expression of GnRH-R in rat germinal cells, testis and pituitary gland was determined by Northern blot analysis using the same mouse-specific probe. Three receptor transcripts were detected in all cases. In mouse pituitary, ovary and testis, we found two GnRH-R transcripts in the proximity of 4.6-4.7 and 3.4 kb, as well as a 1.6 kb transcript in the pituitary and a 2.0-2.1 kb transcript in both the ovary and testis. Mouse germ cells also exhibited three GnRH-R transcripts of 4.7, 3.5 and 2.2 kb. Two distinct GnRH-R transcripts were also detected in the rat pituitary (4.6 and 2.1 kb), testis (4.7 and 3.5 kb) and germ cells (4.5 and 3.5 kb). In addition, a third transcript was detected in rat pituitary (1.9 kb) and in rat testis and germinal cells (2.1 kb). The present study demonstrates that GnRH-R mRNA is expressed in rat and mouse testicular germ cells. We suggest that GnRH-R present in these cells may interact with GnRH or GnRH-like peptides produced in the testis and may be part of a paracrine system. The presence of multiple GnRH-R encoding transcripts is also of interest and warrants further studies to evaluate their regulation and function.
引用
收藏
页码:582 / 586
页数:5
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