CircRNA profiling identifies circRNF180 as a tumor suppressor in hepatocellular carcinoma

被引：8

作者：

Zeng, Xi ^{[1
,2
]}

Tan, Chao ^{[3
,4
]}

Mo, Meile ^{[3
]}

Qin, Xiaoling ^{[5
]}

Ma, Xiaoyun ^{[3
]}

Huang, Kaiyong ^{[1
]}

Wang, Xiaomin ^{[3
]}

Liang, Wenjie ^{[6
]}

Yang, Li ^{[1
]}

机构：

[1] Guangxi Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, Nanning, Peoples R China

[2] Guilin Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, Guilin, Peoples R China

[3] Guangxi Med Univ, Sch Publ Hlth, Dept Epidemiol & Hlth Stat, Nanning, Peoples R China

[4] Guilin Med Univ, Sch Publ Hlth, Dept Epidemiol & Stat, Guilin, Peoples R China

[5] Guangxi Univ Chinese Med, Sch Publ Hlth & Management, Dept Biostat, Nanning, Peoples R China

[6] Guangxi Med Univ, Sch Publ Hlth, Dept Social Med, Nanning, Peoples R China

来源：

EPIGENOMICS | 2021年 / 13卷 / 07期

关键词：

circRNF180; circRNA; hepatocellular carcinoma; RNA sequencing; tumor suppressor; CIRCULAR RNAS; MICROVASCULAR INVASION; VASCULAR INVASION; EXPRESSION; DATABASE; PROGRESSION; METASTASIS; SURVIVAL;

D O I：

10.2217/epi-2020-0385

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: To explore the expression profiles and functions of circRNAs in hepatocellular carcinoma (HCC). Materials & methods: We obtained circRNA expression profiles through RNA sequencing. Expression levels of circRNAs were confirmed by quantitative real-time PCR. The effects on HCC progression were determined using Cell Counting Kit 8, clone formation and transwell assays. Results: We identified 114 upregulated and 144 downregulated circRNAs in HCC tissues. The results of quantitative real-time PCR showed that circGNAO1, circRNF180 and circMERTK were significantly downregulated in HCC tissues, whereas circSNX6 was significantly upregulated. CircRNF180 was associated with microvascular invasion. Overexpression of circRNF180 inhibits the proliferation, colony formation, migration and invasion of HCC cells. Conclusion: CircRNF180 may function as a tumor suppressor and could serve as a potential biomarker and therapeutic target in HCC.

引用

页码：513 / 530

页数：18

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