Terrimonas suqianensis sp nov., isolated from a tetrabromobisphenol A-contaminated soil

Strain C3-5(T), a Gram-negative, asporogenous, rod-shaped bacterium, was isolated from a tetrabromobisphenol A contaminated soil. Growth was observed at 10-37 A degrees C (optimum 30 A degrees C) and at pH 5.5-9.5 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain C3-5(T) is a member of the genus Terrimonas and exhibits high sequence similarities with Terrimonas pekingensis QH(T) (99.0%) and Terrimonas rhizosphaerae CR94(T) (97.3%), and exhibits low (< 97%) sequence similarities with other known Terrimonas species. Chemotaxonomic analysis revealed that strain C3-5(T) possesses menaquinone-7 (MK-7) as the major isoprenoid quinone and iso-C-15:0, iso-C-15:1 G, iso-C-17:0 3-OH and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) as the major (> 5% of total) fatty acids. The polar lipids were determined to be a lipid, glycolipid, phospholipid, phosphoaminolipid and phosphatidylethanolamine. The DNA G+C content was found to be 42.6 mol%. The DNA-DNA relatedness values with the closely related strains T. pekingensis QH(T) and T. rhizosphaerae CR94(T) were 25.2 and 48.5%, respectively. Based on phylogenetic analysis, phenotypic characteristics and chemotaxonomic data, strain C3-5(T) is considered to represent a novel species of the genus Terrimonas, for which the name Terrimonas suqianensis sp. nov. is proposed. The type strain is C3-5(T) (= CCTCC AB 2017042(T) = KCTC 52676(T)).