A Potential SARS-CoV-2 Variant of Interest (VOI) Harboring Mutation E484K in the Spike Protein Was Identified within Lineage B.1.1.33 Circulating in Brazil

被引：25

作者：

Resende, Paola Cristina ^{[1
,2
]}

Graf, Tiago ^{[1
,3
]}

Paixao, Anna Carolina Dias ^{[1
,2
]}

Appolinario, Luciana ^{[1
,2
]}

Lopes, Renata Serrano ^{[1
,2
]}

Mendonca, Ana Carolina da Fonseca ^{[1
,2
]}

da Rocha, Alice Sampaio Barreto ^{[1
,2
]}

Motta, Fernando Couto ^{[1
,2
]}

Neto, Lidio Goncalves Lima ^{[4
]}

Khouri, Ricardo ^{[1
,3
,5
]}

de Oliveira, Camila I. ^{[1
,3
,5
]}

Santos-Muccillo, Pedro ^{[1
,3
,6
]}

Bezerra, Joao Felipe ^{[7
]}

Teixeira, Dalane Loudal Florentino ^{[8
]}

Riediger, Irina ^{[9
]}

Debur, Maria do Carmo ^{[9
]}

Ribeiro-Rodrigues, Rodrigo ^{[10
,11
]}

Leite, Anderson Brandao ^{[12
]}

do Santos, Cliomar Alves ^{[13
]}

Gregianini, Tatiana Schaffer ^{[14
]}

Fernandes, Sandra Bianchini ^{[15
]}

Bernardes, Andre Felipe Leal ^{[16
]}

Cavalcanti, Andrea Cony ^{[17
]}

Miyajima, Fabio ^{[1
,18
]}

Sachhi, Claudio ^{[19
]}

Mattos, Tirza ^{[20
]}

da Costa, Cristiano Fernandes ^{[21
]}

Delatorre, Edson ^{[1
,22
]}

Wallau, Gabriel L. ^{[1
,23
,24
]}

Naveca, Felipe G. ^{[1
,25
]}

Bello, Gonzalo ^{[1
,26
]}

Siqueira, Marilda Mendonca ^{[1
,2
]}

机构：

[1] Fiocruz MS, Fiocruz COVID 19 Genom Surveillance Network, BR-21040360 Rio De Janeiro, Brazil

[2] Fiocruz MS, Oswaldo Cruz Inst, Lab Resp Viruses & Measles LVRS, BR-21045900 Rio De Janeiro, Brazil

[3] Fiocruz BA, Inst Goncalo Moniz, Plataforma Vigilancia Mol, BR-40296710 Salvador, BA, Brazil

[4] Lab Cent Saude Publ Estado Maranhao LACEN MA, BR-65020904 Sao Luis, Maranhao, Brazil

[5] Fiocruz BA, Inst Goncalo Moniz, Lab Enfermidades Infecciosas Transmitidas Vetores, BR-40296710 Salvador, BA, Brazil

[6] Fiocruz BA, Lab Patol & Biol Mol, Inst Goncalo Moniz, BR-40296710 Salvador, BA, Brazil

[7] Univ Fed Paraiba, UFPB, Lab Vigilancia Mol Aplicada, Escola Tecn Saude,Ctr Ciencias Saude, BR-58051900 Joao Pessoa, Paraiba, Brazil

[8] Lab Cent Saude Publ Estado Paraiba LACEN PB, BR-58013360 Joao Pessoa, Paraiba, Brazil

[9] Lab Cent Saude Publ Estado Parana LACEN PR, BR-83060500 Sao Jose Dos Pinhais, Brazil

[10] Lab Cent Saude Publ Estado Espirito Santo LACEN E, BR-29050755 Vitoria, ES, Brazil

[11] Univ Fed Espirito Santo, Nucleo Doencas Infecciosas, BR-29050625 Vitoria, ES, Brazil

[12] Lab Cent Saude Publ Estado Alagoas LACEN AL, BR-57036860 Maceio, Alagoas, Brazil

[13] Lab Cent Saude Publ Estado Sergipe LACEN SE, BR-49020380 Aracaju, Brazil

[14] Lab Cent Saude Publ Estado Rio Grande Sul LACEN R, BR-90610000 Porto Alegre, RS, Brazil

[15] Lab Cent Saude Publ Estado Santa Catarina LACEN S, BR-88010002 Florianopolis, SC, Brazil

[16] Lab Cent Saude Publ Estado Minas Gerais LACEN MG, BR-30510010 Belo Horizonte, MG, Brazil

[17] Lab Cent Saude Publ Estado Rio de Janeiro LACEN R, BR-20231092 Rio De Janeiro, Brazil

[18] Fiocruz MS, Branch Ceara, Oswaldo Cruz Fdn, BR-61760000 Eusebio, Brazil

[19] Inst Adolfo Lutz IAL, BR-01246000 Sao Paulo, Brazil

[20] Lab Cent Saude Publ Amazonas, BR-69020040 Manaus, Amazonas, Brazil

[21] Fundacao Vigilancia Saude Amazonas, BR-69060000 Manaus, Amazonas, Brazil

[22] Univ Fed Espirito Santo, Ctr Ciencias Exatas Nat & Saude, Dept Biol, BR-29500000 Alegre, Brazil

[23] FIOCRUZ PE, Inst Aggeu Magalhaes IAM, Dept Entomol, BR-50740465 Recife, PE, Brazil

[24] FIOCRUZ PE, Inst Aggeu Magalhaes IAM, Nucleo Bioinformat, BR-50740465 Recife, PE, Brazil

[25] Fiocruz AM, Leonidas & Maria Deane Inst, Lab Ecol Doencas Transmissiveis Amazonia EDTA, BR-69057070 Manaus, Amazonas, Brazil

[26] Fiocruz MS, Inst Oswaldo Cruz, Lab AIDS & Imunol Mol, BR-21040900 Rio De Janeiro, Brazil

来源：

VIRUSES-BASEL | 2021年 / 13卷 / 05期

关键词：

SARS-CoV-2; E484K; variant of Interest; genomic epidemiology; Brazil;

D O I：

10.3390/v13050724

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epidemic in Brazil was dominated by two lineages designated as B.1.1.28 and B.1.1.33. The two SARS-CoV-2 variants harboring mutations at the receptor-binding domain of the Spike (S) protein, designated as lineages P.1 and P.2, evolved from lineage B.1.1.28 and are rapidly spreading in Brazil. Lineage P.1 is considered a Variant of Concern (VOC) because of the presence of multiple mutations in the S protein (including K417T, E484K, N501Y), while lineage P.2 only harbors mutation S:E484K and is considered a Variant of Interest (VOI). On the other hand, epidemiologically relevant B.1.1.33 deriving lineages have not been described so far. Here we report the identification of a new SARS-CoV-2 VOI within lineage B.1.1.33 that also harbors mutation S:E484K and was detected in Brazil between November 2020 and February 2021. This VOI displayed four non-synonymous lineage-defining mutations (NSP3:A1711V, NSP6:F36L, S:E484K, and NS7b:E33A) and was designated as lineage N.9. The VOI N.9 probably emerged in August 2020 and has spread across different Brazilian states from the Southeast, South, North, and Northeast regions.

引用

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