Fluorescence-lifetime imaging of DNA-dye interactions within continuous-flow microfluidic systems

被引：25
|
作者：
Benninger, Richard K. P.
Hofmann, Oliver
Onfelt, Bjorn
Munro, Ian
Dunsby, Chris
Davis, Daniel M.
Neil, Mark A. A.
French, Paul M. W.
de Mello, Andrew J.
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, London SW7 2AZ, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England
[3] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 13期
基金：
英国工程与自然科学研究理事会; 英国医学研究理事会;
关键词：
fluorescence; imaging; intercalation; microfluidics; microreactors;
D O I：
10.1002/anie.200604112
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Light across the channel: The utility of time-resolved fluorescence imaging as a detection modality for microfluidic systems has been extended by a study of DNA-dye binding interactions. The combination of time-resolved fluorescence imaging and continuous-flow microfluidics provides a powerful route to the resolution and quantification of multistate biological systems with precise spatial and temporal reaction control. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：2228 / 2231
页数：4
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