Fluorescence-lifetime imaging of DNA-dye interactions within continuous-flow microfluidic systems

被引：25

作者：

Benninger, Richard K. P.

Hofmann, Oliver

Onfelt, Bjorn

Munro, Ian

Dunsby, Chris

Davis, Daniel M.

Neil, Mark A. A.

French, Paul M. W.

de Mello, Andrew J.

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[3] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 13期

基金：

英国工程与自然科学研究理事会; 英国医学研究理事会;

关键词：

fluorescence; imaging; intercalation; microfluidics; microreactors;

D O I：

10.1002/anie.200604112

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Light across the channel: The utility of time-resolved fluorescence imaging as a detection modality for microfluidic systems has been extended by a study of DNA-dye binding interactions. The combination of time-resolved fluorescence imaging and continuous-flow microfluidics provides a powerful route to the resolution and quantification of multistate biological systems with precise spatial and temporal reaction control. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2228 / 2231

页数：4

共 32 条

[1] Fluorescence-lifetime imaging of DNA-Dye interactions within continuous-flow microfluidic systems (vol 46, pg 8525, 2007)
Benninger, R. K. P.
Hofmann, O.
Onfelt, B.
Munro, I.
Dunsby, C.
Davis, D. M.
Neil, M. A. A.
French, P. M. W.
de Mello, A. J.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (45) : 8536 - 8536
[2] Fluorescence lifetime imaging of mixing dynamics in continuous-flow microdroplet reactors
Srisa-Art, Monpichar
deMello, Andrew J.
Edel, Joshua B.
PHYSICAL REVIEW LETTERS, 2008, 101 (01)
[3] Experimental and simulated fluorescence depolarization due to energy transfer as tools to study DNA-dye interactions
Carlsson, C
Larsson, A
Bjorkman, M
Jonsson, M
Albinsson, B
BIOPOLYMERS, 1997, 41 (05) : 481 - 494
[4] Experimental and simulated fluorescence depolarization due to energy transfer as tools to study DNA-dye interactions
Department of Physical Chemistry, Chalmers University of Technology, S-412 96 Goteborg, Sweden
BIOPOLYMERS, 5 (481-494):
[5] Circumventing air bubbles in microfluidic systems and quantitative continuous-flow PCR applications
Nakayama, Tsuyoshi
Kurosawa, Yasunori
Furui, Satoshi
Kerman, Kagan
Kobayashi, Masaaki
Rao, S. Ramachandra
Yonezawa, Yuji
Nakano, Kouichi
Hino, Akihiro
Yamamura, Shohei
Takamura, Yuzuru
Tamiya, Eiichi
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 386 (05) : 1327 - 1333
[6] Circumventing air bubbles in microfluidic systems and quantitative continuous-flow PCR applications
Tsuyoshi Nakayama
Yasunori Kurosawa
Satoshi Furui
Kagan Kerman
Masaaki Kobayashi
S. Ramachandra Rao
Yuji Yonezawa
Kouichi Nakano
Akihiro Hino
Shohei Yamamura
Yuzuru Takamura
Eiichi Tamiya
Analytical and Bioanalytical Chemistry, 2006, 386 : 1327 - 1333
[7] THE USE OF SOLVENT SEGMENTATION IN CONTINUOUS-FLOW SYSTEMS, AND FLUORESCENCE LABELING BY DERIVATIZATION
WERKHOVENGOEWIE, CE
BRINKMAN, UAT
FREI, RW
ANALYTICA CHIMICA ACTA, 1980, 114 (FEB) : 147 - 154
[8] DNA analysis microfluidic chip using continuous-flow PCR and CE-ECD
Joung, Seung-Ryong
Seo, Il
Lee, Hyun Ho
Kang, C. J.
Kim, Yong-Sang
MICROPROCESSES AND NANOTECHNOLOGY 2007, DIGEST OF PAPERS, 2007, : 338 - 339
[9] Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets
Schaerli, Yolanda
Wootton, Robert C.
Robinson, Tom
Stein, Viktor
Dunsby, Christopher
Neil, Mark A. A.
French, Paul M. W.
deMello, Andrew J.
Abell, Chris
Hollfelder, Florian
ANALYTICAL CHEMISTRY, 2009, 81 (01) : 302 - 306
[10] Fast and continuous-flow separation of DNA-complexes and topological DNA variants in microfluidic chip format
Viefhues, Martina
Regtmeier, Jan
Anselmetti, Dario
ANALYST, 2013, 138 (01) : 186 - 196

← 1 2 3 4 →