Interferon alpha-inducible protein 27 promotes epithelial-mesenchymal transition and induces ovarian tumorigenicity and stemness

被引：67

作者：

Li, Shuqin ^{[1
]}

Xie, Yan ^{[2
]}

Zhang, Wei ^{[3
]}

Gao, Junfeng ^{[4
]}

Wang, Man ^{[1
]}

Zheng, Guoxuan ^{[1
]}

Yin, Xing ^{[1
]}

Xia, Hongping ^{[5
]}

Tao, Xiang ^{[6
]}

机构：

[1] Wannan Med Coll, Yijishan Hosp, Dept Obstet & Gynecol, Wuhu 241001, Peoples R China

[2] Yangpu Dist Cent Hosp, Dept Obstet & Gynecol, Shanghai, Peoples R China

[3] Wannan Med Coll, Yijishan Hosp, Dept Pathol, Wuhu 241001, Peoples R China

[4] Hefei First Peoples Hosp, Dept Oncol, Hefei, Peoples R China

[5] Wannan Med Coll, Yijishan Hosp, Dept Surg, Wuhu 241001, Peoples R China

[6] Fudan Univ, Dept Pathol, Obstet & Gynecol Hosp, Shanghai 200433, Peoples R China

来源：

JOURNAL OF SURGICAL RESEARCH | 2015年 / 193卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Ovarian cancer; Metastasis; Epithelial-mesenchymal transition; IFI27; miR-502; SINGLE-NUCLEOTIDE POLYMORPHISM; BREAST-CANCER; SET8; GENE; EXPRESSION; REGION; SITE; IDENTIFICATION; CARCINOMA;

D O I：

10.1016/j.jss.2014.06.055

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Background: Interferon alpha-inducible protein 27 (IFI27) is an interferon alpha-inducible protein, which was found to be upregulated in some cancers, such as breast cancer, squamous cell carcinoma, hepatocellular carcinoma, and serous ovarian carcinoma. However, the role of IFI27 in ovarian cancer remains to be elucidated. This study was designed to investigate the role of IFI27 in ovarian cancer tumorigenicity. Materials and methods: The expression of IFI27 was examined in ovarian cancer tissues and cell lines by real time quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The cell migration and invasion was investigated by wound healing and transwell invasion assay. The epithelial-mesenchymal transition markers were detected by Western blotting and the stemness was evaluated by sphere formation. The tumor growth was examined in the athymic mice model. Results: We found that IFI27 is overexpressed in ovarian cancer and associated with patients' survival. Interestingly, we further observed that the expression of IFI27 was associated with the expression of mesenchymal marker vimentin in ovarian cancer. Overexpression of IFI27 induces epithelial-mesenchymal transition and promotes epithelial ovarian cancer cells migration and invasion, tumorigenicity, stemness, and drug resistance. Moreover, overexpression of IFI27 is associated with loss of miR-502 in ovarian cancer. Reexpression of miR-502 inhibits IFI27-induced tumorigenicity, migration, and drug resistance. Conclusions: These data suggested that IFI27 may be a potential target for developing novel diagnosis strategies and therapeutic interventions. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：255 / 264

页数：10

共 25 条

[1] Integrated genomic analyses of ovarian carcinoma [J].

Bell, D. ;

Berchuck, A. ;

Birrer, M. ;

Chien, J. ;

Cramer, D. W. ;

Dao, F. ;

Dhir, R. ;

DiSaia, P. ;

Gabra, H. ;

Glenn, P. ;

Godwin, A. K. ;

Gross, J. ;

Hartmann, L. ;

Huang, M. ;

Huntsman, D. G. ;

Iacocca, M. ;

Imielinski, M. ;

Kalloger, S. ;

Karlan, B. Y. ;

Levine, D. A. ;

Mills, G. B. ;

Morrison, C. ;

Mutch, D. ;

Olvera, N. ;

Orsulic, S. ;

Park, K. ;

Petrelli, N. ;

Rabeno, B. ;

Rader, J. S. ;

Sikic, B. I. ;

Smith-McCune, K. ;

Sood, A. K. ;

Bowtell, D. ;

Penny, R. ;

Testa, J. R. ;

Chang, K. ;

Dinh, H. H. ;

Drummond, J. A. ;

Fowler, G. ;

Gunaratne, P. ;

Hawes, A. C. ;

Kovar, C. L. ;

Lewis, L. R. ;

Morgan, M. B. ;

Newsham, I. F. ;

Santibanez, J. ;

Reid, J. G. ;

Trevino, L. R. ;

Wu, Y. -Q. ;

Wang, M. .

NATURE, 2011, 474 (7353) :609-615

[2] Induction of a unique gene expression profile in primary human hepatocytes by hepatitis C virus core, NS3 and NS5A proteins [J].

Budhu, A. ;

Chen, Y. ;

Kim, J. W. ;

Forgues, M. ;

Valerie, K. ;

Harris, C. C. ;

Wang, X. W. .

CARCINOGENESIS, 2007, 28 (07) :1552-1560

[3] A polymorphism at the miR-502 binding site in the 3′ untranslated region of the SET8 gene is associated with the outcome of small-cell lung cancer [J].

Ding, Cuimin ;

Li, Ruijuan ;

Peng, Jingcui ;

Li, Shengmian ;

Guo, Zhanjun .

EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2012, 3 (04) :689-692

[4] A polymorphism at the miR-502 binding site in the 3′-untranslated region of the histone methyltransferase SET8 is associated with hepatocellular carcinoma outcome [J].

Guo, Zhanjun ;

Wu, Chensi ;

Wang, Xiaoling ;

Wang, Cuiju ;

Zhang, Ruixing ;

Shan, Baoen .

INTERNATIONAL JOURNAL OF CANCER, 2012, 131 (06) :1318-1322

[5]

He L., 2004, NAT REV GENET, V5, P522

[6] Recent Progress in the Diagnosis and Treatment of Ovarian Cancer [J].

Jelovac, Danijela ;

Armstrong, Deborah K. .

CA-A CANCER JOURNAL FOR CLINICIANS, 2011, 61 (03) :183-203

[7] Identification of differentially expressed genes using an annealing control primer system in stage III serous ovarian carcinoma [J].

Kim, Yun-Sook ;

Hwan, Jin Do ;

Bae, Sumi ;

Bae, Dong-Han ;

Shick, Woong Ahn .

BMC CANCER, 2010, 10

[8]

Kong Dejuan, 2011, Cancers (Basel), V3, P716, DOI 10.3390/cancers30100716

[9] Current status and evolution of preclinical drug development models of epithelial ovarian cancer [J].

Konstantinopoulos, Panagiotis A. ;

Matulonis, Ursula A. .

FRONTIERS IN ONCOLOGY, 2013, 3

[10] Molecular profiling supports the role of epithelial-to-mesenchymal transition (EMT) in ovarian cancer metastasis [J].

Lili, Loukia N. ;

Matyunina, Lilya V. ;

Walker, L. DeEtte ;

Wells, Stephen L. ;

Benigno, Benedict B. ;

McDonald, John F. .

JOURNAL OF OVARIAN RESEARCH, 2013, 6

← 1 2 3 →