Photocatalytic Regeneration of Nicotinamide Cofactors by Quantum Dot-Enzyme Biohybrid Complexes

被引：94

作者：

Brown, Katherine A. ^{[1
]}

Wilker, Molly B. ^{[2
]}

Boehm, Marko ^{[1
]}

Hamby, Hayden ^{[2
]}

Dukovic, Gordana ^{[2
]}

King, Paul W. ^{[1
]}

机构：

[1] Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 USA

[2] Univ Colorado, Dept Chem & Biochem, Campus Box 215, Boulder, CO 80309 USA

来源：

ACS CATALYSIS | 2016年 / 6卷 / 04期

关键词：

biohybrid; NADPH regeneration; photocatalysis; biocatalysis; quantum dots; BIOCATALYTIC REDOX REACTIONS; ELECTROCHEMICAL REGENERATION; VISIBLE-LIGHT; ELECTRON-TRANSFER; HYDROGENASE COMPLEXES; NADH; CDS; SEMICONDUCTOR; FERREDOXIN; REDUCTION;

D O I：

10.1021/acscatal.5b02850

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report the characterization of biohybrid complexes of CdSe quantum dots and ferredoxin NADP(-)(+)reductase for photocatalytic regeneration of NADPH. Illumination with visible light led to reduction of NADP+ to NADPH, with an apparent k(cat) of 1400 h(-1). Regeneration of NADPH was coupled to reduction of aldehydes to alcohols catalyzed by a NADPH-dependent alcohol dehydrogenase, Aldehyde with each NADPH molecule recycled an average of 7.5 times. The quantum yield both of NADPH and alcohol production were 5-6% for both products. Light-driven NADPH regeneration was also demonstrated in a multienzyme system, showing the capacity of QD-FNR complexes to drive continuous NADPH-dependent transformations.

引用

页码：2201 / 2204

页数：4

共 30 条

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