Specific SR protein-dependent splicing substrates identified through genomic SELEX

被引：55

作者：

Kim, S ^{[1
]}

Shi, H ^{[1
]}

Lee, D ^{[1
]}

Lis, JT ^{[1
]}

机构：

[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

来源：

NUCLEIC ACIDS RESEARCH | 2003年 / 31卷 / 07期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/gkg286

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Drosophila pre-mRNA splicing factor B52 (SRp55) is essential for fly development, but splicing of RNAs of specific genes tested previously is normal in B52-null animals, presumably due to partial functional redundancy with other SR proteins. To identify B52-dependent splicing substrates in vivo, we selected genomic sequence fragments whose transcripts bind B52. Almost all of the corresponding genes having a known function encode either transcription factors or components of signal transduction pathways, with the B52-binding fragments located to not only exonic but also intronic regions. Some pre-mRNAs from these genes showed splicing defects in the B52-null mutant. These results indicate that B52 has unique functions in the removal of some introns during development, and plays a critical role in cellular regulatory networks.

引用

页码：1955 / 1961

页数：7

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