Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations

被引：108

作者：

Chen, Ya-Wen ^{[1
]}

Song, Shilin ^{[1
]}

Weng, Ruifen ^{[1
]}

Verma, Pushpa ^{[1
]}

Kugler, Jan-Michael ^{[1
]}

Buescher, Marita ^{[1
]}

Rouam, Sigrid ^{[3
]}

Cohen, Stephen M. ^{[1
,2
]}

机构：

[1] Inst Mol & Cell Biol, Singapore 138673, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[3] Procter & Gamble Int Operat SA Singapore Branch, Quantitat Sci, Stat Asia, Singapore 138547, Singapore

来源：

DEVELOPMENTAL CELL | 2014年 / 31卷 / 06期

关键词：

MUSHROOM BODY NEURONS; PARKINSONS-DISEASE; CELL-PROLIFERATION; GENE-EXPRESSION; BANTAM MIRNA; C-ELEGANS; LIFE-SPAN; ENDS-OUT; PATHWAY; EVOLUTION;

D O I：

10.1016/j.devcel.2014.11.029

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.

引用

页码：784 / 800

页数：17

共 87 条

[1] The let-7 microRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans [J].