Phenolic Profile and Biological Activities of the Pepino (Solanum muricatum) Fruit and Its Wild Relative S. caripense

被引:27
作者
Herraiz, Francisco J. [1 ]
Villano, Debora [2 ]
Plazas, Mariola [1 ]
Vilanova, Santiago [1 ]
Ferreres, Federico [2 ]
Prohens, Jaime [1 ]
Moreno, Diego A. [2 ]
机构
[1] Univ Politecn Valencia, Inst Conservac & Mejora Agrodiversidad Valenciana, Camino Vera 14, E-46022 Valencia, Spain
[2] CSIC, Ctr Edafol & Biol Aplicada Segura, Dept Food Sci & Technol, Res Grp Qual Safety & Bioact Plant Foods, Campus Univ Espinardo 25, Murcia 30100, Spain
关键词
antioxidants; macrophages; biological activity; HPLC-DAD-MSn/ESI; DIFFERENT VARIETAL TYPES; LC-MSN IDENTIFICATION; ANTIOXIDANT CAPACITY; CHLOROGENIC ACIDS; GENETIC-RESOURCES; MASS-SPECTROMETRY; IN-VITRO; EGGPLANT; SOLANACEAE; VEGETABLES;
D O I
10.3390/ijms17030394
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pepino (Solanum muricatum) is an edible and juicy fruit native to the Andean region which is becoming increasingly important. However, little information is available on its phenolic composition and bioactive properties. Four pepino varieties (37-A, El Camino, Puzol, and Valencia) and one accession (E-7) of its close wild relative S. caripense were characterized by HPLC-DAD-MSn/ESI. Twenty-four hydroxycinnamic acid derivatives were detected (5 to 16 compounds per variety or accession), with differences of more than two-fold for their total content among the materials studied. The major phenolics in the pepino varieties were chlorogenic acids and derivatives, while in S. caripense a caffeoyl-synapoyl-quinic acid was the major compound. The in vitro antioxidant capacity (DPPH (2,2-diphenyl-1-picrylhydrazyl hydrate), ORAC (oxygen radical absorbance capacity), and TRC (total reducing capacity) tests) was higher in S. caripense. Pepino and S. caripense extracts were not toxic for RAW 264.7 macrophage cells, and the raw extracts inhibited NO production of the lipopolysaccharide (LPS)-stimulated macrophages by 36% (El Camino) to 67% (37-A). No single variety ranked high simultaneously for hydroxycinnamic acids content, antioxidant activity and biological activity. We suggest the screening of large collections of germplasm or the use of complementary crosses between Puzol (high for hydroxycinnamic acids and biological activity) and S. caripense E-7 (high for antioxidant activity) to select and breed pepino varieties with enhanced properties.
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页数:15
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