HMGB1 interacts differentially with members of the Rel family of transcription factors

被引：86
作者：
Agresti, A
Lupo, R
Bianchi, ME
Müller, S
机构：
[1] DIBIT, San Raffaele Sci Inst, I-20132 Milan, Italy
[2] Univ Vita Salute San Raffaele, I-20132 Milan, Italy
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 302卷 / 02期
关键词：
HMGB1; Rel proteins; NF-kappa B; protein-protein interaction; transcription;
D O I：
10.1016/S0006-291X(03)00184-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
HMGB1 is an architectural factor that enhances the DNA binding affinity of several proteins. We have investigated the influence of HMGB1 on DNA binding by members of the Rel family. HMGB1 enhances DNA binding by p65/p50 and p50/p50, but reduces binding by p65/p65, c-Rel/c-Rel, p65/c-Rel, and p50/c-Rel. In pull-down assays, HMGB1 interacts directly with the p50 subunit via its HMG boxes and this interaction is weakened by the presence of the acidic tail. Functionally, HMGB1 is required for the NF-KB-dependent expression of the adhesion molecule VCAM-1. (C) 2003 Elsevier Science (USA). All rights reserved.
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收藏
页码：421 / 426
页数：6
相关论文
共 28 条
[1]   The NF-kappa B and I kappa B proteins: New discoveries and insights [J].
Baldwin, AS .
ANNUAL REVIEW OF IMMUNOLOGY, 1996, 14 :649-683
[2]   Upwardly mobile proteins Workshop: The role of HMG proteins in chromatin structure, gene expression and neoplasia [J].
Bianchi, Marco E. ;
Beltrame, Monica .
EMBO REPORTS, 2000, 1 (02) :109-114
[3]   THE DNA-BINDING SITE OF HMG1 PROTEIN IS COMPOSED OF 2 SIMILAR SEGMENTS (HMG BOXES), BOTH OF WHICH HAVE COUNTERPARTS IN OTHER EUKARYOTIC REGULATORY PROTEINS [J].
BIANCHI, ME ;
FALCIOLA, L ;
FERRARI, S ;
LILLEY, DMJ .
EMBO JOURNAL, 1992, 11 (03) :1055-1063
[4]   High-mobility group chromatin proteins 1 and 2 functionally interact with steroid hormone receptors to enhance their DNA binding in vitro and transcriptional activity in mammalian cells [J].
Boonyaratanakornkit, V ;
Melvin, V ;
Prendergast, P ;
Altmann, M ;
Ronfani, L ;
Bianchi, ME ;
Taraseviciene, L ;
Nordeen, SK ;
Allegretto, EA ;
Edwards, DP .
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (08) :4471-4487
[5]   Interactions between an HMG-1 protein and members of the Rel family [J].
Brickman, JM ;
Adam, M ;
Ptashne, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (19) :10679-10683
[6]   Revised nomenclature for high mobility group (HMG) chromosomal proteins [J].
Bustin, M .
TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (03) :152-153
[7]  
Bustin M, 1999, MOL CELL BIOL, V19, P5237
[8]   The lack of chromosomal protein Hmg1 does not disrupt cell growth but causes lethal hypoglycaemia in newborn mice [J].
Calogero, S ;
Grassi, F ;
Aguzzi, A ;
Voigtländer, T ;
Ferrier, P ;
Ferrari, S ;
Bianchi, ME .
NATURE GENETICS, 1999, 22 (03) :276-280
[9]   The Drosophila DSP1 gene encoding an HMG 1-like protein: Genomic organization, evolutionary conservation and expression [J].
Canaple, L ;
Decoville, M ;
Leng, M ;
Locker, D .
GENE, 1997, 184 (02) :285-290
[10]   Regulation of DNA binding by Rel/NF-κB transcription factors:: structural views [J].
Chen, FE ;
Ghosh, G .
ONCOGENE, 1999, 18 (49) :6845-6852
123