piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

被引：12

作者：

Wolff, Jonas Holst ^{[1
]}

Haldrup, Jakob ^{[1
]}

Thomsen, Emil Aagaard ^{[1
]}

Andersen, Sofie ^{[1
]}

Mikkelsen, Jacob Giehm ^{[1
]}

机构：

[1] Aarhus Univ, Dept Biomed, Aarhus, Denmark

来源：

FRONTIERS IN GENOME EDITING | 2021年 / 3卷

关键词：

prime editing; CRISPR; prime editing guide RNA; pegRNA; piggyBac; DNA transposition; KNOCKOUT;

D O I：

10.3389/fgeed.2021.786893

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.

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页数：7

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