piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

被引：10

作者：

Wolff, Jonas Holst ^{[1
]}

Haldrup, Jakob ^{[1
]}

Thomsen, Emil Aagaard ^{[1
]}

Andersen, Sofie ^{[1
]}

Mikkelsen, Jacob Giehm ^{[1
]}

机构：

[1] Aarhus Univ, Dept Biomed, Aarhus, Denmark

来源：

FRONTIERS IN GENOME EDITING | 2021年 / 3卷

关键词：

prime editing; CRISPR; prime editing guide RNA; pegRNA; piggyBac; DNA transposition; KNOCKOUT;

D O I：

10.3389/fgeed.2021.786893

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.

引用

页数：7

共 21 条

[1] pegIT - a web-based design tool for prime editing
Anderson, Mads Valdemar
Haldrup, Jakob
Thomsen, Emil Aagaard
Wolff, Jonas Holst
Mikkelsen, Jacob Giehm
[J]. NUCLEIC ACIDS RESEARCH, 2021, 49 (W1) : W505 - W509
[2] Search-and-replace genome editing without double-strand breaks or donor DNA
Anzalone, Andrew V.
Randolph, Peyton B.
Davis, Jessie R.
Sousa, Alexander A.
Koblan, Luke W.
Levy, Jonathan M.
Chen, Peter J.
Wilson, Christopher
Newby, Gregory A.
Raguram, Aditya
Liu, David R.
[J]. NATURE, 2019, 576 (7785) : 149 - +
[3] Deconvolution of Complex DNA Repair (DECODR): Establishing a Novel Deconvolution Algorithm for Comprehensive Analysis of CRISPR-Edited Sanger sequencing Data
Bloh, Kevin
Kanchana, Rohan
Bialk, Pawel
Banas, Kelly
Zhang, Zugui
Yoo, Byung-Chun
Kmiec, Eric B.
[J]. CRISPR JOURNAL, 2021, 4 (01): : 120 - 131
[4] Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing
Chemello, F.
Chai, A. C.
Li, H.
Rodriguez-Caycedo, C.
Sanchez-Ortiz, E.
Atmanli, A.
Mireault, A. A.
Liu, N.
Bassel-Duby, R.
Olson, E. N.
[J]. SCIENCE ADVANCES, 2021, 7 (18):
[5] In Vivo Knockout of the Vegfa Gene by Lentiviral Delivery of CRISPR/Cas9 in Mouse Retinal Pigment Epithelium Cells
Holmgaard, Andreas
Askou, Anne Louise
Benckendorff, Josephine Natalia Esther
Thomsen, Emil Aagaard
Cai, Yujia
Bek, Toke
Mikkelsen, Jacob Giehm
Corydon, Thomas J.
[J]. MOLECULAR THERAPY-NUCLEIC ACIDS, 2017, 9 : 89 - 99
[6] Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases
Jang, Hyewon
Jo, Dong Hyun
Cho, Chang Sik
Shin, Jeong Hong
Seo, Jung Hwa
Yu, Goosang
Gopalappa, Ramu
Kim, Daesik
Cho, Sung-Rae
Kim, Jeong Hun
Kim, Hyongbum Henry
[J]. NATURE BIOMEDICAL ENGINEERING, 2022, 6 (02) : 181 - +
[7] RNA-programmed genome editing in human cells
Jinek, Martin
East, Alexandra
Cheng, Aaron
Lin, Steven
Ma, Enbo
Doudna, Jennifer
[J]. ELIFE, 2013, 2
[8] A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, Martin
Chylinski, Krzysztof
Fonfara, Ines
Hauer, Michael
Doudna, Jennifer A.
Charpentier, Emmanuelle
[J]. SCIENCE, 2012, 337 (6096) : 816 - 821
[9] Adenine base editing and prime editing of chemically derived hepatic progenitors rescue genetic liver disease
Kim, Yohan
Hong, Sung-Ah
Yu, Jihyeon
Eom, Jeongyun
Jang, Kiseok
Yoon, Sangtae
Hong, Da Hee
Seo, Daekwan
Lee, Seu-Na
Woo, Jae-Sung
Jeong, Jaemin
Bae, Sangsu
Choi, Dongho
[J]. CELL STEM CELL, 2021, 28 (09) : 1614 - +
[10] Systematic determination of the packaging limit of lentiviral vectors
Kumar, M
Keller, B
Makalou, N
Sutton, RE
[J]. HUMAN GENE THERAPY, 2001, 12 (15) : 1893 - 1905

← 1 2 3 →