The Glc2Man2-fragment of the N-glycan precursor - a novel ligand for the glycan-binding protein malectin?

被引：9

作者：

Mueller, Lisa N. ^{[1
]}

Muhle-Goll, Claudia ^{[2
]}

Biskup, Moritz B. ^{[1
]}

机构：

[1] KIT, Inst Organ Chem, D-76131 Karlsruhe, Germany

[2] KIT, Inst Biol Grenzflachen IBG 2, D-76021 Karlsruhe, Germany

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2010年 / 8卷 / 14期

关键词：

L-FUCOPYRANOSYL GROUPS; ENDOPLASMIC-RETICULUM; CARBOHYDRATE MOIETY; COMPLEX TYPE; GLYCOPROTEINS; GLYCOSIDES; OLIGOSACCHARIDES; TETRASACCHARIDE; DISACCHARIDES; SACCHARIDE;

D O I：

10.1039/c004502k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The Glc alpha(1 -> 3)Glc alpha(1 -> 3)Man alpha(1 -> 2) Man tetrasaccharide (Glc(2)Man(2)-fragment), a substructure of the natural N-glycan precursor, was synthesized. The interaction of this fragment with the protein malectin, a carbohydrate binding protein localized in the endoplasmatic reticulum, was investigated by (HN)-H-1-N-15 HSQC experiments and isothermal calorimetry. The chemical shift perturbations of nuclei in the protein's backbone caused by the binding of the Glc(2)Man(2)-fragment to malectin suggest a binding mode like the known ligand nigerose.

引用

页码：3294 / 3299

页数：6

共 33 条

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