Hydrophobic Triaryl-Substituted β-Lactams as Activity-Based Probes for Profiling Eukaryotic Enzymes and Host-Pathogen Interactions

被引：11

作者：

Nasheri, Neda ^{[1
,2
]}

McKay, Craig S. ^{[1
,3
]}

Fulton, Kelly ^{[1
]}

Twine, Susan ^{[1
]}

Powdrill, Megan H. ^{[1
,3
]}

Sherratt, Allison R. ^{[1
]}

Pezacki, John Paul ^{[1
,2
,3
]}

机构：

[1] Natl Res Council Canada, Div Life Sci, Ottawa, ON K1A 0R6, Canada

[2] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada

[3] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada

来源：

CHEMBIOCHEM | 2014年 / 15卷 / 15期

关键词：

activity-based protein profiling; beta-lactams; eukaryote; hepatitis C virus; mass spectrometry; HEPATITIS-C-VIRUS; HEPATOCELLULAR-CARCINOMA; PROTEOMIC ANALYSIS; CELL-CYCLE; PROTEIN; REPLICATION; RESISTANCE; ANTIBIOTICS; MODULATION; MECHANISMS;

D O I：

10.1002/cbic.201402097

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ABPP with β-lactams: We identified the eukaryotic targets of β-lactam-containing compounds by activity-based protein profiling. Using this method, we demonstrated that β-lactam-based activity probes can be applied to identify differentially active enzymes in different cell lines and during hepatitis C virus replication. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：2195 / 2200

页数：6

共 2 条

[1] Activity-Based Protein Profiling at the Host-Pathogen Interface
Kovalyova, Yekaterina
Hatzios, Stavroula K.
ACTIVITY-BASED PROTEIN PROFILING, 2019, 420 : 73 - 91
[2] Pyrethroid activity-based probes for profiling cytochrome P450 activities associated with insecticide interactions
Ismail, Hanafy M.
O'Neill, Paul M.
Hong, David W.
Finn, Robert D.
Henderson, Colin J.
Wright, Aaron T.
Cravatt, Benjamin F.
Hemingway, Janet
Paine, Mark J. I.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (49) : 19766 - 19771

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