Major Carotenoids in Tobacco Laminas: Identification and Quantification by HPLC with Photodiode Array Detection

被引:0
作者
Chu, Dayan [1 ]
Xie, Yueqin [1 ]
Hong, Shenqiu [1 ]
Yan, Xiangyang [1 ]
Tong, Hongwu [1 ]
Liu, Shaomin [1 ]
机构
[1] Univ Sci & Technol China, Res Ctr Tobacco & Hlth, Hefei 230052, Anhui, Peoples R China
关键词
Carotenoids; Tobacco; HPLC; Quantification; Identification; VEGETABLES; LEAVES;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The carotenoid compositions in various tobacco laminas are determined by high-performance liquid chromatography (HPLC) with the photodiode array detection (DAD). The carotenoids are extracted from powdered tobacco laminas by cold acetone containing 0.1 % butylated hydroxytoluene (BHT). A mobile phase of CH3CN-H2O (90:10, V/V) (A) and CH3COOC2H5 (100 %) (B) with following gradient elution is developed: 100 % A in the beginning, maintained for 20 min, decrease to 50 % A in 50 min, maintain for 5 min and return to 100 % A in I h. A total of I I carotenoids including some isomers in tobacco laminas are resolved within 55 min by using a ZorBAX SB-C-18 column with the flow-rate at 1.0 mL/min and detection at 445 nm. The limits of detection (LODs) of carotenoids varies from 6.0 to 14.0 ng/mL. The relative standard deviations (RSD) are from 2.69 to 3.63 % and the recovery ranges from 89.6 to 96.3 %. The method is successfully applied for the quantification of carotenoids in green, fresh flue-cured and aging tobacco laminas and the analysis results show that total carotenoids in tobacco laminas range from 49.66 to 820.1 mu g/g. Lutein and beta-carotene are the most representative carotenoids in flue-cured and aging tobacco laminas because other carotenoids degrade greatly during flue-curing and aging periods'. Furthermore, it worth to mention that zeaxanthin is not found in green tobacco laminas while presented in fresh flue-cured and aging tobacco laminas, luteoxanthin as a new carotenoid is first tentatively identified in the present work and alpha-carotene is not found in any significant amount in all those tobacco laminas.
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页码:2635 / 2647
页数:13
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