A fluorogenic dye activated by the Staudinger ligation

被引：163

作者：

Lemieux, GA

de Graffenried, CL

Bertozzi, CR ^{[1
]}

机构：

[1] Univ Calif Berkeley, Ctr New Direct Organ Synth, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Ctr New Direct Organ Synth, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 16期

关键词：

D O I：

10.1021/ja029013y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Specific labeling of biomolecules with biochemical and biophysical probes is a central element of proteomics research. Here we describe a coumarin-phosphine dye that undergoes activation of coumarin fluorescence upon Staudinger ligation with azides. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications. Copyright © 2003 American Chemical Society.

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页码：4708 / 4709

页数：2

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