The skeletal muscle satellite cell response to a single bout of resistance-type exercise is delayed with aging in men

被引:101
作者
Snijders, Tim [1 ]
Verdijk, Lex B. [1 ]
Smeets, Joey S. J. [1 ]
McKay, Bryon R. [2 ]
Senden, Joan M. G. [1 ]
Hartgens, Fred [3 ,4 ]
Parise, Gianni [2 ]
Greenhaff, Paul [5 ]
van Loon, Luc J. C. [1 ]
机构
[1] Maastricht Univ, Fac Hlth Med & Life Sci, Dept Human Movement Sci, NL-6200 MD Maastricht, Netherlands
[2] McMaster Univ, Dept Kinesiol, Hamilton, ON, Canada
[3] Maastricht Univ, Dept Epidemiol, NL-6200 MD Maastricht, Netherlands
[4] Maastricht Univ, Dept Surg, NL-6200 MD Maastricht, Netherlands
[5] Univ Nottingham, Queens Med Ctr, MRC ARUK Ctr Musculoskeletal Ageing Res, Nottingham NG7 2RD, England
关键词
Muscle stem cells; Myostatin; MyoD; Myogenin; OLDER MEN; LENGTHENING CONTRACTIONS; PROTEIN SUPPLEMENTATION; MYOFIBER HYPERTROPHY; MYOSTATIN EXPRESSION; MYONUCLEAR ADDITION; ECCENTRIC EXERCISE; GAMMA-IRRADIATION; PROLONGED ABSENCE; GENE-EXPRESSION;
D O I
10.1007/s11357-014-9699-z
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
Skeletal muscle satellite cells (SCs) have been shown to be instrumental in the muscle adaptive response to exercise. The present study determines agerelated differences in SC content and activation status following a single bout of exercise. Ten young (22 +/- 1 years) and 10 elderly (73 +/- 1 years) men performed a single bout of resistance-type exercise. Muscle biopsies were collected before and 12, 24, 48, and 72 h after exercise. SC content and activation status were assessed in type I and type II muscle fibers by immunohistochemistry. Myostatin and MyoD protein and messenger RNA (mRNA) expression were determined by Western blotting and rtPCR, respectively. In response to exercise, it took 48 h (young) and 72 h (elderly) for type II muscle fiber SC content to exceed baseline values (P<0.01). The number of myostatin+ SC in type I and II muscle fibers was significantly reduced after 12, 24, and 48 h of post-exercise recovery in both groups (P< 0.01), with a greater reduction observed at 24 and 48 h in the young compared with that in the elderly men (P< 0.01). In conclusion, the increase in type II muscle fiber SC content during post-exercise recovery is delayed with aging and is accompanied by a blunted SC activation response.
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页数:15
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