Transcript accumulation of carotenoid biosynthesis genes in the cyanobacterium Synechocystis sp PCC 6803 during the dark-to-light transition is mediated by photosynthetic electron transport

被引:4
作者
Ryu, Jee-Youn [1 ]
Song, Ji-Young [1 ]
Chung, Youngho [2 ]
Park, Young-Mok [3 ,5 ]
Chow, Wah Soon [4 ]
Park, Youn-Il [1 ,5 ]
机构
[1] Chungnam Natl Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Taejon 305764, South Korea
[2] Korea Basic Sci Inst, Proteome Anal Team, Taejon 305333, South Korea
[3] Korea Basic Sci Inst, Mass Spectrometer Res Ctr, Taejon 305333, South Korea
[4] Australian Natl Univ, Res Sch Biol, Div Plant Sci, Coll Med Biol & Environm, Canberra, ACT 0200, Australia
[5] Chungnam Natl Univ, Grad Sch Analyt Sci & Technol, Taejon 305764, South Korea
关键词
Carotenoid biosynthesis gene; Light induction; Photoreceptor; Photosynthetic electron transport; Synechocystis; PLECTONEMA-BORYANUM; GROWTH IRRADIANCE; PHYTOENE SYNTHASE; PHOTOSYSTEM-II; REDOX CONTROL; SP PCC-6803; EXPRESSION; PROTEIN; PHYTOCHROME; PLASTOQUINONE;
D O I
10.1007/s11816-010-0130-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Expression of the genes for carotenoid biosynthesis (crt) is dependent on light, but little is known about the underlying mechanism of light sensing and signalling in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter, Synechocystis). In the present study, we investigated the light-induced increase in the transcript levels of Synechocystis crt genes, including phytoene synthase (crtB), phytoene desaturase (crtP), zeta-carotene desaturase (crtQ), and beta-carotene hydroxylase (crtR), during a dark-to-light transition period. During the dark-to-light shift, the increase in the crt transcript levels was not affected by mutations in cyanobacterial photoreceptors, such as phytochromes (cph1, cph2 and cph3) and a cryptochrome-type photoreceptor (ccry), or respiratory electron transport components NDH and Cyd/CtaI. However, treatment with photosynthetic electron transport inhibitors significantly diminished the accumulation of crt gene transcripts. Therefore, the light induction of the Synechocystis crt gene expression is most likely mediated by photosynthetic electron transport rather than by cyanobacterial photoreceptors during the dark-to-light transition.
引用
收藏
页码:149 / 155
页数:7
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