The effect of dietary polyphenols on the epigenetic regulation of gene expression in MCF7 breast cancer cells

被引:143
|
作者
Paluszczak, Jaroslaw [1 ]
Krajka-Kuzniak, Violetta [1 ]
Baer-Dubowska, Wanda [1 ]
机构
[1] Poznan Univ Med Sci, Dept Pharmaceut Biochem, PL-60781 Poznan, Poland
关键词
Chemoprevention; DNA methylation; Epigenetics; Histone methylation; Polyphenols; CATECHOL-O-METHYLTRANSFERASE; DNA METHYLATION; INHIBITION; GENISTEIN; HYPERMETHYLATION; LOCALIZATION; MODULATION;
D O I
10.1016/j.toxlet.2009.10.010
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
The CpG island methylator phenotype is characterized by DNA hypermethylation in the promoters of several suppressor genes associated with the inactivation of various pathways involved in tumorigenesis. DNA methylation is catalyzed by specific DNA methyltransferases (DNMTs). Dietary phytochemicals particularly catechol-containing polyphenols were shown to inhibit these enzymes and reactivate epigenetically silenced genes. The aim of this study was to evaluate the effect of a wide range of dietary phytochemicals on the activity and expression of DNMTs in human breast cancer MCF7 cell line and their effect on DNA and histone H3 methylation. All phytochemicals inhibited the DNA methyltransferase activity with betanin being the weakest while rosmarinic and ellagic acids were the most potent modulators (up to 88% inhibition). While decitabine led to a partial demethylation and reactivation of the genes, none of the tested phytochemicals affected the methylation pattern or the expression of RASSF1A, GSTP1 or HIN1 in MCF7 cells. The global methylation of histone H3 was not affected by any of the tested phytochemicals or decitabine. The results of our study may suggest that non-nucleoside agents are not likely to be effective epigenetic modulators, in our experimental model at least. However, a long-term exposure to these chemicals in diet might potentially lead to an effect, which can be sufficient for cancer chemoprevention. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:119 / 125
页数:7
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