NINA-LAMP compared to microscopy, RDT, and nested PCR for the detection of imported malaria

被引：36

作者：

Mohon, Abu Naser ^{[1
,2
]}

Lee, Lydia Da-Yeong ^{[1
]}

Bayih, Abebe Genetu ^{[2
]}

Folefoc, Asongna ^{[2
]}

Guelig, Dylan ^{[3
]}

Burton, Robert A. ^{[3
]}

LaBarre, Paul ^{[3
]}

Chan, Wilson ^{[2
]}

Meatherall, Bonnie

Pillai, Dylan R. ^{[1
,2
,4
]}

机构：

[1] Univ Calgary, Dept Microbiol Immunol & Infect Dis, Calgary, AB, Canada

[2] Univ Calgary, Dept Pathol & Lab Med, Calgary, AB, Canada

[3] PATH, Seattle, WA USA

[4] Univ Calgary, Dept Med, Calgary, AB, Canada

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2016年 / 85卷 / 02期

关键词：

Malaria; Point of care test; LAMP; MEDIATED ISOTHERMAL AMPLIFICATION; RAPID DIAGNOSTIC-TESTS; VISITING FRIENDS; PERFORMANCE; SURVEILLANCE; SENSITIVITY; TRAVELERS; ASSAY; BINAX; GENE;

D O I：

10.1016/j.diagmicrobio.2015.11.009

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Microscopy and field adaptable rapid diagnostic tests (RDTs) are not sensitive and specific in certain conditions such as poor training of microscopists, lack of electricity, or lower sensitivity in the detection of non falciparum malaria. More sensitive point-of-care testing (POCT) would reduce delays in diagnosis and initiation of therapy. In the current study, we have evaluated the efficacy of noninstrumented nucleic acid amplification (NINA) coupled with loop-mediated isothermal amplification (LAMP) for detection of traveler's malaria (n = 140) in comparison with microscopy, nested PCR, and the only Food and Drug Administration-approved rapid diagnostic test. NINA-LAMP was 100% sensitive and 98.6% specific when compared to nested PCR. For non falciparum detection, NINA-LAMP sensitivity was 100% sensitive compared to nested PCR, whereas RDT sensitivity was 71%. LAMP is highly sensitive and specific for symptomatic malaria diagnosis regardless of species. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：149 / 153

页数：5

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