Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels

被引：4
作者：
Cervantes-Salguero, Keitel [1 ]
Kawamata, Ibuki [1 ]
Nomura, Shin-ichiro M. [1 ]
Murata, Satoshi [1 ]
机构：
[1] Tohoku Univ, Grad Sch Engn, Dept Robot, 6-6-1 Aobayama, Sendai, Miyagi 9808579, Japan
来源：
PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2017年 / 19卷 / 21期
关键词：
GOLD NANOPARTICLES; NUCLEIC-ACIDS; SINGLE; IMMOBILIZATION; NANOTECHNOLOGY; HYBRIDIZATION; MUTATIONS; INTEGRIN; FORCES;
D O I：
10.1039/c7cp02214j
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm(-1)) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.
引用
收藏
页码：13414 / 13418
页数：5
相关论文
共 35 条
[1]   Electrophoretic Mobility of Colloidal Gold Particles in Electrolyte Solutions [J].
Agnihotri, Sagar M. ;
Ohshima, Hiroyuki ;
Terada, Hiroshi ;
Tomoda, Keishiro ;
Makino, Kimiko .
LANGMUIR, 2009, 25 (08) :4804-4807
[2]   DNA Nanotechnology for Precise Control over Drug Delivery and Gene Therapy [J].
Angell, Chava ;
Xie, Sibai ;
Zhang, Liangfang ;
Chen, Yi .
SMALL, 2016, 12 (09) :1117-1132
[3]   What are the emerging concepts and challenges in NANO? Nanoarchitectonics, hand-operating nanotechnology and mechanobiology [J].
Ariga, Katsuhiko ;
Minami, Kosuke ;
Ebara, Mitsuhiro ;
Nakanishi, Jun .
POLYMER JOURNAL, 2016, 48 (04) :371-389
[4]   Programmed folding of DNA origami structures through single-molecule force control [J].
Bae, Wooli ;
Kim, Kipom ;
Min, Duyoung ;
Ryu, Je-Kyung ;
Hyeon, Changbong ;
Yoon, Tae-Young .
NATURE COMMUNICATIONS, 2014, 5
[5]   Hydrogel porosity controlling DNA-directed immobilization of gold nanoparticles revealed by DNA melting and scanning helium ion microscopy [J].
Baeissa, Ajfan ;
Moghimi, Nafiseh ;
Liu, Juewen .
RSC ADVANCES, 2012, 2 (07) :2981-2987
[6]   Crowding-Induced Hybridization of Single DNA Hairpins [J].
Baltierra-Jasso, Laura E. ;
Morten, Michael J. ;
Laflor, Linda ;
Quinn, Steven D. ;
Magennis, Steven W. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (51) :16020-16023
[7]   Assembly of metal nanoparticles into nanogaps [J].
Barsotti, Robert J., Jr. ;
Vahey, Michael D. ;
Wartena, Ryan ;
Chiang, Yet-Ming ;
Voldman, Joel ;
Stellacci, Francesco .
SMALL, 2007, 3 (03) :488-499
[8]   Mechanisms for initiating cellular DNA replication [J].
Bleichert, Franziska ;
Botchan, Michael R. ;
Berger, James M. .
SCIENCE, 2017, 355 (6327)
[9]   Grabbing the cat by the tail: manipulating molecules one by one [J].
Bustamante C. ;
Macosko J.C. ;
Wuite G.J.L. .
Nature Reviews Molecular Cell Biology, 2000, 1 (2) :130-136
[10]  
Chirieleison SM, 2013, NAT CHEM, V5, P1000, DOI [10.1038/NCHEM.1764, 10.1038/nchem.1764]
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