Polypyrrole/polylactic acid nanofibrous scaffold cotransplanted with bone marrow stromal cells promotes the functional recovery of spinal cord injury in rats

被引:36
作者
Raynald [1 ,2 ]
Shu, Bing [3 ]
Liu, Xue-Bin [2 ]
Zhou, Jun-Feng [4 ,5 ]
Huang, Hua [6 ]
Wang, Jing-Yun [4 ,5 ]
Sun, Xiao-Dan [4 ,5 ]
Qin, Chuan [1 ]
An, Yi-Hua [2 ,3 ]
机构
[1] CAMS, Comparat Med Ctr, PUMC, Inst Lab Anim Sci, Beijing, Peoples R China
[2] Chinese PLA Peoples Liberat Army Gen Hosp, Med Ctr 3, Dept Funct Neurosurg, Beijing 100039, Peoples R China
[3] Capital Med Univ, Beijing Sanbo Brain Hosp, Dept Neurosurg, Beijing, Peoples R China
[4] Tsinghua Univ, Sch Mat Sci & Engn, State Key Lab New Ceram & Fine Proc, Beijing, Peoples R China
[5] Tsinghua Univ, Sch Mat Sci & Engn, Minist Educ China, Key Lab Adv Mat, Beijing, Peoples R China
[6] Capital Med Univ, Beijing Neurosurg Inst, Beijing, Peoples R China
基金
中国国家自然科学基金; 英国科研创新办公室;
关键词
bone marrow stromal cell; functional recovery; PPy; PLA nanofibrous scaffold; spinal cord injury; transplantation; ELECTRICAL-STIMULATION; STEM-CELLS; GLIAL SCAR; GROWTH; REGENERATION; GRAFTS; REPAIR;
D O I
10.1111/cns.13135
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Aims The objective of this study was to analyze the efficacy of polypyrrole/polylactic acid (PPy/PLA) nanofibrous scaffold cotransplanted with bone marrow stromal cells (BMSCs) in promoting the functional recovery in a rat spinal cord injury (SCI). Methods Female Sprague-Dawley rats were randomly divided into three groups (n = 18/group): control group, PPy/PLA group, and PPy/PLA/BMSCs group. The SCI was induced in all rats. Consequently, rats in PPy/PLA/BMSCs group were transplanted with 1 x 10(5) BMSCs after implantation of PPy/PLA, while those in the PPy/PLA group were implanted with PPy/PLA only; no implantation was performed in the control group. Six weeks after surgery, immunofluorescence microscopy, electron microscope, and polymerase chain reaction (PCR) techniques were performed to assess the changes in the injured spinal cord tissues. Results Electrophysiology and locomotor function testing suggested that PPy/PLA nanofibrous scaffold cotransplanted with BMSCs could promote the functional recovery of the spinal cord. Six weeks after the operation, lower amount of scar tissue was found in the PPy/PLA group compared with the control group. Abundant neurofilament (NF) and neuron-specific marker (NeuN) positive staining, and myelin formations were detected in the injured area. In addition, the transplantation of BMSCs not only improved the efficacy of PPy/PLA but also managed to survive well and was differentiated into neural and neuroglial cells. Conclusions The implantation of PPy/PLA nanofibrous scaffold and BMSCs has a great potential to restore the electrical conduction and to promote functional recovery by inhibiting the scar tissue formation, promoting axon regeneration, and bridging the gap lesion.
引用
收藏
页码:951 / 964
页数:14
相关论文
共 39 条
[1]   Tissue engineering of peripheral nerves: A comparison of venous and acellular muscle grafts with cultured Schwann cells [J].
Fansa, H ;
Keilhoff, G ;
Wolf, G ;
Schneider, W .
PLASTIC AND RECONSTRUCTIVE SURGERY, 2001, 107 (02) :485-494
[2]   The glial scar and central nervous system repair [J].
Fawcett, JW ;
Asher, RA .
BRAIN RESEARCH BULLETIN, 1999, 49 (06) :377-391
[3]   Schwann cell response on polypyrrole substrates upon electrical stimulation [J].
Forciniti, Leandro ;
Ybarra, Jose, III ;
Zaman, Muhammad H. ;
Schmidt, Christine E. .
ACTA BIOMATERIALIA, 2014, 10 (06) :2423-2433
[4]   From angiogenesis to neuropathology [J].
Greenberg, DA ;
Jin, KL .
NATURE, 2005, 438 (7070) :954-959
[5]   Neural tissue engineering options for peripheral nerve regeneration [J].
Gu, Xiaosong ;
Ding, Fei ;
Williams, David F. .
BIOMATERIALS, 2014, 35 (24) :6143-6156
[6]   Conducting polymers in biomedical engineering [J].
Guimard, Nathalie K. ;
Gomez, Natalia ;
Schmidt, Christine E. .
PROGRESS IN POLYMER SCIENCE, 2007, 32 (8-9) :876-921
[7]   Electrical Stimulation Accelerates Axonal and Functional Peripheral Nerve Regeneration across Long Gaps [J].
Haastert-Talini, Kirsten ;
Schmitte, Ruth ;
Korte, Nele ;
Klode, Dorothee ;
Ratzka, Andreas ;
Grothe, Claudia .
JOURNAL OF NEUROTRAUMA, 2011, 28 (04) :661-674
[8]   Electrical stimulation to promote osteogenesis using conductive polypyrrole films [J].
Hu, Wei-Wen ;
Hsu, Yi-Ting ;
Cheng, Yu-Che ;
Li, Chuan ;
Ruaan, Ruoh-Chyu ;
Chien, Chih-Cheng ;
Chung, Chih-Ang ;
Tsao, Chia-Wen .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2014, 37 :28-36
[9]   Electrical Stimulation Accelerates Motor Functional Recovery in the Rat Model of 15-mm Sciatic Nerve Gap Bridged by Scaffolds With Longitudinally Oriented Microchannels [J].
Huang, Jinghui ;
Lu, Lei ;
Hu, Xueyu ;
Ye, Zhengxu ;
Peng, Ye ;
Yan, Xiaodong ;
Geng, Dan ;
Luo, Zhuojing .
NEUROREHABILITATION AND NEURAL REPAIR, 2010, 24 (08) :736-745
[10]   Robust CNS regeneration after complete spinal cord transection using aligned poly-L-lactic acid microfibers [J].
Hurtado, Andres ;
Cregg, Jared M. ;
Wang, Han B. ;
Wendell, Dane F. ;
Oudega, Martin ;
Gilbert, Ryan J. ;
McDonald, John W. .
BIOMATERIALS, 2011, 32 (26) :6068-6079