α1-adrenergic stimulation potentiates the thermogenic action of β3-adrenoreceptor-generated cAMP in brown fat cells

The relationship between cAMP levels and thermogenesis was investigated in brown fat cells from Syrian hamsters, Irrespective of whether the selective beta(3)-, beta(2)-, and beta(1)-agonists BRL 37344, salbutamol, and dobutamine or the physiological agonist norepinephrine was used to stimulate the cells, increases in cAMP levels were mediated via the beta(3)-receptor, as were the thermogenic effects, However, the relationship "thermogenesis per cAMP" was much lower for agents other than norepinephrine, Similarly, forskolin, although more potent than norepinephrine in elevating cAMP, was less potent in inducing thermogenesis, The selective alpha(1)-agonist cirazoline was in itself without effect on cAMP levels or thermogenesis, but when added to forskolin-stimulated cells, potentiated thermogenesis, up to the norepineph rine level, without affecting cAMP. This potentiation could not be inhibited by chelerythrine, but could be mimicked by Ca2+ ionophores, It was apparently not mediated via calmodulin-dependent protein kinase and was not an effect on mitochondrial respiratory control, The ability of all cAMP-elevating agents to induce thermogenesis in brown fat cells has earlier been interpreted to mean that it is only through the beta-receptors and the resulting increase in cAMP levels that thermogenesis is induced, However, it is here concluded that the thermogenic response to norepinephrine involves two interacting parts, one mediated via beta-receptors and cAMP and the other via alpha(1)-receptors and increases in cytosolic Ca2+ levels.