Anti-inflammatory and antiallergic activity of arbutin against ovalbumin-induced allergic rhinitis in mice through the modulation of inflammatory responses

Background: Allergic rhinitis (AR) is the prevalent inflammatory disease in the airway due to allergic reactions in response to numerous allergens. AR is considered as a type-I allergic condition and proceeded as early- and late-phase hypersensitivity. Objectives: In the existing work, we scheduled to discover the anti-inflammatory potential of arbutin against the ovalbumin (OVA)-provoked AR in mice through modulation of inflammation. Materials and Methods: The AR was triggered to the BALB/c mice by injecting 500 mu L of OVA sensitization solution and then treated with the arbutin (25 and 50 mg/kg, respectively). Dexamethasone was used as standard. The occurrences of sneezing and nasal rubbing were detected within 15 min after the last OVA challenge. The status of OVA-specific immunoglobulin E (IgE), histamine, and malondialdehyde (MDA) was scrutinized by using assay kits. The status of NF-kappa B/I kappa B alpha and STAT-3 signaling molecules and its related cytokines was considered by using assay kits. The histological scores were evaluated by the histological alterations. Results: The arbutin supplementation lessened the incidence of nasal rubbings and sneezing, OVA-specific IgE and histamine, and MDA status. The arbutin-administered AR mice established the appreciable reduction in the NF-kappa Bp65, phosphorylated I kappa B alpha, interleukin (IL)-1 beta, and tumor necrosis factor-alpha levels, also improved the I kappa B alpha status in the AR mice. Arbutin reduced the STAT-3, phosphorylated STAT-3, RORc, IL-17A, IL-5, and IL-6 levels and elevated the IL-10, IL-12, and IFN-gamma status. Arbutin supplementation to the AR mice exhibited the considerable amelioration of the histological scores of OVA-provoked AR mice. Conclusion: Our results established that the arbutin treatment effectively ameliorated the OVA-provoked AR in mice through the modulation of inflammation, and it could be auspicious therapeutic agent to treat the RA.