LncRNA MT1JP acts as a tumor inhibitor via reciprocally regulating Wnt/β-Catenin pathway in retinoblastoma

被引：3

作者：

Bi, L. -L. ^{[1
]}

Han, F. ^{[2
]}

Zhang, X. -M. ^{[3
]}

Li, Y. -Y. ^{[2
]}

机构：

[1] Mudanjiang Coll Med, Affiliated Hosp 2, Dept Med Engn, Mudanjiang, Peoples R China

[2] Mudanjiang Coll Med, Affiliated Hosp 2, Dept Ophthalmol, Mudanjiang, Peoples R China

[3] Mudanjiang Coll Med, Dept Lib, Mudanjiang, Peoples R China

来源：

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES | 2018年 / 22卷 / 13期

关键词：

Retinoblastoma; MT1JP; beta-catenn signaling; EMT; LONG NONCODING RNAS; HUMAN BREAST-CANCER; CELL LUNG-CANCER; SIGNALING PATHWAY; INTRAARTERIAL CHEMOTHERAPY; CERVICAL-CANCER; PROLIFERATION; METASTASIS; INVASION; ADENOCARCINOMA;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

OBJECTIVE: The aim of this study was to investigate the roles of MT1JP and beta-catenin in retinoblastoma. PATIENTS AND METHODS: We performed quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) to quantify the expressions of MT1JP and beta-catenin in 44 retinoblastoma tissues and matched non-tumor tissues. What's more. retinoblastoma cell lines were transfected with pcDNA3.1-MT1JP, after which proliferation, cell cycle, apoptosis, and expression of beta-catenin as well as its downstream targets were assayed. We also conducted TOP-Flash reporter assay to explore the activity of Wnt/beta-catenin signaling pathway. RESULTS: The results revealed that MT1JP was down-regulated, while beta-catenin was highly expressed in retinoblastoma cells. Meanwhile, the forced expression of MT1JP impaired the expression of the beta-catenin protein and its downstream targets such as cyclin D1. c-myc. CONCLUSIONS: We demonstrated that MT1JP was a tumor suppressor by negatively modulating the activity of the Wnt/beta-catenin signaling pathway in the development of retinoblastoma and might function as a prognostic biomarker and therapeutic target.

引用

页码：4204 / 4214

页数：11