Long non-coding RNA lnc-GNAT1-1 inhibits gastric cancer cell proliferation and invasion through the Wnt/-catenin pathway in Helicobacter pylori infection

被引：23
作者：
Liu, Liping [1 ,2 ]
Shuai, Tiankui [3 ]
Li, Bin [2 ]
Zhu, Lei [2 ]
Li, Xun [1 ]
机构：
[1] Lanzhou Univ, Hosp 1, Dept Crit Care Med, 1 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China
[2] Lanzhou Univ, Hosp 1, Dept Crit Care Med, Donggang Branch, Lanzhou 730000, Gansu, Peoples R China
[3] Lanzhou Univ, Hosp 1, Dept Gen Surg, Lanzhou 730000, Gansu, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2018年 / 18卷 / 04期
关键词：
long non-coding RNA; Helicobacter pylori; gastric cancer; Wnt; -catenin pathway; SIGNALING PATHWAY; OVEREXPRESSION; THERAPY;
D O I：
10.3892/mmr.2018.9405
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim of the present study was to investigate the effect of long non-coding (lnc)-G protein subunit transducin 1 (GNAT1)-1 in gastric cancer cells infected with Helicobacter (H.) pylori. Reverse transcription-quantitative polymerase chain reaction was used to assess lnc-GNAT1-1 expression in normal gastric and gastric cancer cells infected with H. pylori. The overexpression of lnc-GNAT1-1 in SGC-7901 and MNK45 cells was induced by transfection. Migration and invasion assays were performed in transfected gastric cancer cells to evaluate the effect of lnc-GNAT1-1. Histological and western blot analyses were used to determine the alterations in the Wnt/-catenin signaling pathway protein expression. Cells transfected with lnc-GNAT1-1 were used to initiate gastric cancer tumor xenografts to compare tumor growth in mice inoculated with untransfected cells. The results revealed that H. pylori infection significantly downregulated lnc-GNAT-1 expression. Lnc-GNAT1-1 overexpression inhibited gastric cancer cell migration and invasion. Wnt/-catenin pathway protein expression was decreased by lnc-GNAT1-1 overexpression. In addition, lnc-GNAT1-1 overexpression reduced tumor growth. Thus, lnc-GNAT1-1 is downregulated in gastric cancer infected with H. pylori and lnc-GNAT1-1 overexpression inhibited gastric cancer growth through the Wnt/-catenin signaling pathway.
引用
收藏
页码：4009 / 4015
页数：7
相关论文
共 23 条
[1]   Comprehensive molecular characterization of gastric adenocarcinoma [J].
Bass, Adam J. ;
Thorsson, Vesteinn ;
Shmulevich, Ilya ;
Reynolds, Sheila M. ;
Miller, Michael ;
Bernard, Brady ;
Hinoue, Toshinori ;
Laird, Peter W. ;
Curtis, Christina ;
Shen, Hui ;
Weisenberger, Daniel J. ;
Schultz, Nikolaus ;
Shen, Ronglai ;
Weinhold, Nils ;
Keiser, David P. ;
Bowlby, Reanne ;
Sipahimalani, Payal ;
Cherniack, Andrew D. ;
Getz, Gad ;
Liu, Yingchun ;
Noble, Michael S. ;
Pedamallu, Chandra ;
Sougnez, Carrie ;
Taylor-Weiner, Amaro ;
Akbani, Rehan ;
Lee, Ju-Seog ;
Liu, Wenbin ;
Mills, Gordon B. ;
Yang, Da ;
Zhang, Wei ;
Pantazi, Angeliki ;
Parfenov, Michael ;
Gulley, Margaret ;
Piazuelo, M. Blanca ;
Schneider, Barbara G. ;
Kim, Jihun ;
Boussioutas, Alex ;
Sheth, Margi ;
Demchok, John A. ;
Rabkin, Charles S. ;
Willis, Joseph E. ;
Ng, Sam ;
Garman, Katherine ;
Beer, David G. ;
Pennathur, Arjun ;
Raphael, Benjamin J. ;
Wu, Hsin-Ta ;
Odze, Robert ;
Kim, Hark K. ;
Bowen, Jay .
NATURE, 2014, 513 (7517) :202-209
[2]   Analysis of long non-coding RNA expression profiles in gastric cancer [J].
Cao, Wei-Jun ;
Wu, Hai-Lu ;
He, Bang-Shun ;
Zhang, Yu-Shu ;
Zhang, Zhen-Yu .
WORLD JOURNAL OF GASTROENTEROLOGY, 2013, 19 (23) :3658-3664
[3]   Gene therapy and DNA delivery systems [J].
Ibraheem, D. ;
Elaissari, A. ;
Fessi, H. .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2014, 459 (1-2) :70-83
[4]   Japanese gastric cancer treatment guidelines 2010 (ver. 3) [J].
Sano T. ;
Kodera Y. .
GASTRIC CANCER, 2011, 14 (02) :113-123
[5]   The benefit of mass eradication of Helicobacter pylori infection: a community-based study of gastric cancer prevention [J].
Lee, Yi-Chia ;
Chen, Tony Hsiu-Hsi ;
Chiu, Han-Mo ;
Shun, Chia-Tung ;
Chiang, Hung ;
Liu, Tzeng-Ying ;
Wu, Ming-Shiang ;
Lin, Jaw-Town .
GUT, 2013, 62 (05) :676-682
[6]   Overexpression of IncRNA H19 enhances carcinogenesis and metastasis of gastric cancer [J].
Li, Hao ;
Yu, Beiqin ;
Li, Jianfang ;
Su, Liping ;
Yan, Min ;
Zhu, Zhenggang ;
Liu, Bingya .
ONCOTARGET, 2014, 5 (08) :2318-2329
[7]   MicroRNAs as potential biomarkers for gastric cancer [J].
Liu, Han-Shao ;
Xiao, Hua-Sheng .
WORLD JOURNAL OF GASTROENTEROLOGY, 2014, 20 (34) :12007-12017
[8]   Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer [J].
Liu, Xiang-hua ;
Sun, Ming ;
Nie, Feng-qi ;
Ge, Ying-bin ;
Zhang, Er-bao ;
Yin, Dan-dan ;
Kong, Rong ;
Xia, Rui ;
Lu, Kai-hua ;
Li, Jin-hai ;
De, Wei ;
Wang, Ke-ming ;
Wang, Zhao-xia .
MOLECULAR CANCER, 2014, 13
[9]   Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].
Livak, KJ ;
Schmittgen, TD .
METHODS, 2001, 25 (04) :402-408
[10]  
Mahady GB, 2002, ANTICANCER RES, V22, P4179
123