Hypothermic oxygenated machine perfusion of the human pancreas for clinical islet isolation: a prospective feasibility study

被引:8
|
作者
Doppenberg, Jason B. [1 ]
Leemkuil, Marjolein [2 ,3 ]
Engelse, Marten A. [1 ]
Krikke, Christina [2 ]
de Koning, Eelco J. P. [1 ]
Leuvenink, Henri G. D. [2 ]
机构
[1] Leiden Univ, Transplantat Ctr, Med Ctr, Leiden, Netherlands
[2] Univ Med Ctr Groningen, Dept Surg, Groningen, Netherlands
[3] Univ Med Ctr Groningen, POB 30-001, NL-9700 RB Groningen, Netherlands
关键词
donation after circulatory death; hypothermic machine perfusion; islet isolation; CARDIAC DEATH; KIDNEY-TRANSPLANTATION; CIRCULATORY DEATH; TECHNICAL ASPECTS; PRESERVATION; DONORS; DONATION; VARIABLES; PORCINE; STATE;
D O I
10.1111/tri.13927
中图分类号
R61 [外科手术学];
学科分类号
摘要
Due to an increasing scarcity of pancreases with optimal donor characteristics, islet isolation centers utilize pancreases from extended criteria donors, such as from donation after circulatory death (DCD) donors, which are particularly susceptible to prolonged cold ischemia time (CIT). We hypothesized that hypothermic machine perfusion (HMP) can safely increase CIT. Five human DCD pancreases were subjected to 6 h of oxygenated HMP. Perfusion parameters, apoptosis, and edema were measured prior to islet isolation. Five human DBD pancreases were evaluated after static cold storage (SCS). Islet viability, and in vitro and in vivo functionality in diabetic mice were analyzed. Islets were isolated from HMP pancreases after 13.4 h [12.9-14.5] CIT and after 9.2 h [6.5-12.5] CIT from SCS pancreases. Histological analysis of the pancreatic tissue showed that HMP did not induce edema nor apoptosis. Islets maintained >90% viable during culture, and an appropriate in vitro and in vivo function in mice was demonstrated after HMP. The current study design does not permit to demonstrate that oxygenated HMP allows for cold ischemia extension; however, the successful isolation of functional islets from discarded human DCD pancreases after performing 6 h of oxygenated HMP indicates that oxygenated HMP may be a useful technology for better preservation of pancreases.
引用
收藏
页码:1397 / 1407
页数:11
相关论文
共 50 条
  • [41] PANCREAS PERFUSION WITH HYPOTHERMIC MACHINE: PANCREAS AND DUODENAL HISTOLOGY UP TO 24 HOURS
    Branchereau, Julien
    Renaudin, Karine
    Cantarovich, Diego
    TRANSPLANT INTERNATIONAL, 2017, 30 : 516 - 516
  • [42] EVALUATION OF THE MECHANISM OF ACTION OF HEPATIC HYPOTHERMIC OXYGENATED MACHINE PERFUSION (HOPE) USING MICRODIALYSIS IN A CLINICAL SETTING
    Patrono, Damiano
    Mazzeo, Anna Teresa
    Roggio, Dorotea
    Catalano, Giorgia
    Rigo, Federica
    Fanelli, Vito
    Romagnoli, Renato
    TRANSPLANTATION, 2020, 104 (09) : S110 - S110
  • [43] Hypothermal Machine Perfusion of Human Donor Pancreata Prior to Islet Isolation: A Pilot Study.
    Engelse, Marten
    Leemkuil, Marjolein
    de Nijs, Maaike
    Ringers, Jan
    Braat, Dries
    Leuvenink, Henry
    Krikke, Christina
    de Koning, Eelco
    TRANSPLANTATION, 2013, 96 (06) : S39 - S39
  • [44] PO2 21% oxygenated hypothermic machine perfusion in kidney transplantation: Any clinical benefit?
    Pravisani, Riccardo
    Baccarani, Umberto
    Molinari, Elena
    Cherchi, Vittorio
    Bacchetti, Stefano
    Terrosu, Giovanni
    Avital, Itzhak
    Ekser, Burcin
    Adani, Gian Luigi
    INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 2022, 45 (08): : 666 - 671
  • [45] A Preliminary Clinical Experience Using Hypothermic Oxygenated Machine Perfusion for Rapid Recovery of Octogenarian Liver Grafts
    Rayar, Michel
    Maillot, Betty
    Bergeat, Damien
    Camus, Christophe
    Houssel-Debry, Pauline
    Sulpice, Laurent
    Meunier, Bernard
    Boudjema, Karim
    PROGRESS IN TRANSPLANTATION, 2019, 29 (01) : 97 - 98
  • [46] Hypothermic oxygenated perfusion in human liver transplantation: meta-analysis of randomized clinical trials
    Xie, Xiaolong
    Zheng, Qianwen
    Li, Kewei
    Xiang, Bo
    BRITISH JOURNAL OF SURGERY, 2024, 111 (01)
  • [47] Improved Normothermic Machine Perfusion After Short Oxygenated Hypothermic Machine Perfusion of Ischemically Injured Porcine Kidneys
    Lignell, Stina
    Lohmann, Stine
    Rozenberg, Kaithlyn M.
    Leuvenink, Henri G. D.
    Pool, Merel B. F.
    Lewis, Kate R.
    Moers, Cyril
    Hunter, James P.
    Ploeg, Rutger J.
    Eijken, Marco
    Moldrup, Ulla
    Krag, Soren
    Baan, Carla C.
    Moller, Bjarne Kuno
    Keller, Anna Krarup
    Jespersen, Bente
    TRANSPLANTATION DIRECT, 2021, 7 (02): : E653
  • [48] A merged protocol of hypothermic oxygenated machine perfusion and normothermic machine perfusion optimizes the reconditioning of marginal donor livers
    Boteon, Y.
    Schlegel, A.
    Laing, R.
    Wallace, L.
    Smith, A.
    Attard, J.
    Neil, D.
    Perera, T.
    Mirza, D. F.
    Isaac, J.
    Muiesan, P.
    Afford, S.
    Mergental, H.
    JOURNAL OF HEPATOLOGY, 2018, 68 : S656 - S657
  • [49] PROSPECTIVE EVALUATION OF 2 TECHNIQUES FOR HUMAN PANCREAS RECOVERY BEFORE ISLET ISOLATION
    LAKEY, JRT
    KIZILISIK, TA
    RAJOTTE, RV
    AO, Z
    WARNOCK, GL
    KNETEMAN, NM
    TRANSPLANTATION PROCEEDINGS, 1994, 26 (02) : 589 - 589
  • [50] Return of the cold: How hypothermic oxygenated machine perfusion is changing liver transplantation
    Risbey, Charles W. G.
    Lau, Ngee-Soon
    Niu, Anita
    Zhang, Wesley B.
    Crawford, Michael
    Pulitano, Carlo
    TRANSPLANTATION REVIEWS, 2024, 38 (03)