Plant regeneration via somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp)

被引：24

作者：

Sreenivasu, K

Malik, SK

Kumar, PA ^{[1
]}

Sharma, RP

机构：

[1] Indian Agr Res Inst, Natl Res Ctr Plant Biotechnol, New Delhi 110012, India

[2] Natl Bur Plant Genet Resources, New Delhi 110012, India

来源：

PLANT CELL REPORTS | 1998年 / 17卷 / 04期

关键词：

pigeonpea; Cajanus cajan; somatic embryogenesis;

D O I：

10.1007/s002990050395

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Efficient plant regeneration via somatic embryogenesis has been developed in pigeonpea. Cotyledon and leaf explants from 10-day-old seedlings produced embryogenic callus and somatic embryos when cultured on Murashige and Skoog (MS) medium supplemented with 10 mu M thidiazuron (TDZ). Subsequent withdrawal of TDZ from the induction medium resulted in the maturation and growth of the embryos into plantlets on MS basal medium. The rooted plantlets were transferred and acclimatized on vermiculite where they showed normal morphological characters.

引用

页码：294 / 297

页数：4

共 21 条

[1] PLANT-REGENERATION FROM LEAF-DISKS OF PEANUT AND PIGEONPEA - INFLUENCE OF BENZYLADENINE, INDOLEACETIC-ACID AND INDOLEACETIC-ACID AMINO-ACID CONJUGATES [J].