Transgene expression in rat fetal brain grafts is maintained for 7 months after ex vivo adenoviral vector-mediated gene transfer

被引：8

作者：

van Esseveldt, KE ^{[1
]}

Hermens, WTJMC ^{[1
]}

Verhaagen, J ^{[1
]}

Boer, GJ ^{[1
]}

机构：

[1] Netherlands Inst Brain Res, Grad Sch Neurosci Amsterdam, NL-1105 AZ Amsterdam ZO, Netherlands

来源：

NEUROSCIENCE LETTERS | 1998年 / 240卷 / 02期

关键词：

adenoviral vector; ex vivo gene transfer; neurotransplantation; suprachiasmatic nucleus; in situ hybridization;

D O I：

10.1016/S0304-3940(97)00934-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Fetal brain tissue fragments containing the suprachiasmatic nucleus were infected with an adenoviral vector containing the marker gene LacZ encoding for beta-galactosidase, and subsequently cultured or transplanted in the third ventricle of SCN-lesioned adult Wistar rats. In previous studies we optimized the infection procedure and characterized the immunological response directed against the viral vector in this model. The present study reports on beta-gal expression for at least 7 months in neuronal and glial cells. Maturation of the transplanted fetal SCN with respect to immunoreactivity for vasoactive intestinal polypeptide and C-terminal propressophysin was not hampered by the viral infection. (C) 1998 Elsevier Science Ireland Ltd.

引用

页码：116 / 120

页数：5

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