Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV-ve Oral Cancer Stem Cells

被引：20
作者：
Bano, Nasreen [1 ]
Yadav, Manisha [1 ]
Das, Bhudev C. [2 ]
机构：
[1] Univ Delhi, Dr BR Ambedkar Ctr Biomed Res, New Delhi, India
[2] Amity Univ, Amity Inst Mol Med & Stem Cell Res, Stem Cell & Canc Res Lab, Noida, India
来源：
FRONTIERS IN ONCOLOGY | 2018年 / 8卷
关键词：
cancer stem cells (CSCs); oral squamous cell carcinoma (OSCC); curcumin; human papillomavirus (HPV); SP cells (side population); stemness; orospheres; micro-RNA (miRNA); NF-KAPPA-B; HUMAN-PAPILLOMAVIRUS; MIR-21; EXPRESSION; CERVICAL-CANCER; UP-REGULATION; SELF-RENEWAL; ACTIVATION; RESISTANCE; TRANSCRIPTION; TRANSPORTER;
D O I：
10.3389/fonc.2018.00412
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Aim: To investigate the role of a herbal antioxidative compound curcumin on cell proliferation, orosphere formation and miRNA-21 expression in HPV16+ve/-ve oral cancer stem cells. Materials and Methods: Oral cancer stem cells were isolated from HPV+ve/HPV-ve oral cancer cell lines by FACS and stemness markers. MTT, spheroid assay and qRT-PCR were employed to examine the effects of curcumin. Results: Curcumin treatment in micromolar concentration (0-50 mu M) demonstrated significant differential inhibition in CSC proliferation, orosphere formation and miRNA-21 expression in a dose dependent manner, the effect being highly pronounced in HPV positive CSCs. Conclusion: The strong and dose-dependent inhibitory effects of curcumin on cell proliferation, stemness and miRNA appear to be due to its chemosensitizing and anticancer effects on OSCC-CSCs.
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页数：6
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