Metagenome changes in the biogas producing community during anaerobic digestion of rice straw

被引：24

作者：

Pore, Soham D. ^{[1
]}

Shetty, Deepa ^{[1
,2
]}

Arora, Preeti ^{[1
]}

Maheshwari, Sneha ^{[1
]}

Dhakephalkar, Prashant K. ^{[1
]}

机构：

[1] MACS Agharkar Res Inst, Bioenergy Grp, Pune, Maharashtra, India

[2] MCE Soc, Abeda Inamdar Senior Coll Arts Sci & Commerce, Pune, Maharashtra, India

来源：

BIORESOURCE TECHNOLOGY | 2016年 / 213卷

关键词：

Sour digester; Healthy digester; Metagenomics; Acidogenesis; Methanogenesis; FERMENTATION; ACIDS; NOV;

D O I：

10.1016/j.biortech.2016.03.045

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The present investigation was undertaken to study the microbial community succession in a sour and healthy digester. Ion torrent next-generation sequencing (NGS)-based metagenomic approach indicated abundance of hydrolytic bacteria and exclusion of methanogens and syntrophic bacteria in sour digester. Functional gene analysis revealed higher abundance of enzymes involved in acidogenesis and lower abundance of enzymes associated with methanogenesis like Methyl coenzyme M-reductase, F420 dependent reductase and Formylmethanofuran dehydrogenase in sour digester. Increased abundance of methanogens (Methanomicrobia) and genes involved in methanogenesis was observed in the restored/healthy digester highlighting revival of pH sensitive methanogenic community. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：50 / 53

页数：4

共 16 条

[1] PURIFICATION + PROPERTIES OF ENZYMES INVOLVED IN PROPIONIC ACID FERMENTATION [J].

ALLEN, SHG ;

WOOD, HG ;

KELLERMEYER, RW ;

STJERNHOLM, RL .

JOURNAL OF BACTERIOLOGY, 1964, 87 (01) :171-&

[2]

[Anonymous], 2005, Standard methods for the examination of water and waste- water

[3] Characterization and transcription of the genes involved in butyrate production in Butyrivibrio fibrisolvens TypeI and II strains [J].

Asanuma, N ;

Ishiwata, M ;

Yoshii, T ;

Kikuchi, M ;

Nishina, Y ;

Hino, T .

CURRENT MICROBIOLOGY, 2005, 51 (02) :91-94

[4] The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: A review [J].

Demirel B. ;

Scherer P. .

Reviews in Environmental Science and Bio/Technology, 2008, 7 (2) :173-190

[5] Selenomonas lipolytica sp. nov., an obligately anaerobic bacterium possessing lipolytic activity [J].

Dighe, AS ;

Shouche, YS ;

Ranade, DR .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :783-791

[6] Volatile fatty acids productions by mesophilic and thermophilic sludge fermentation: Biological responses to fermentation temperature [J].

Hao, Jiuxiao ;

Wang, Hui .

BIORESOURCE TECHNOLOGY, 2015, 175 :367-373

[7] Biogas Production from Protein-Rich Biomass: Fed-Batch Anaerobic Fermentation of Casein and of Pig Blood and Associated Changes in Microbial Community Composition [J].

Kovacs, Etelka ;

Wirth, Roland ;

Maroti, Gergely ;

Bagi, Zoltan ;

Rakhely, Gabor ;

Kovacs, Kornel L. .

PLOS ONE, 2013, 8 (10)

[8] Megasphaera indica sp nov., an obligate anaerobic bacteria isolated from human faeces [J].

Lanjekar, V. B. ;

Marathe, N. P. ;

Ramana, V. Venkata ;

Shouche, Y. S. ;

Ranade, D. R. .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2014, 64 :2250-2256

[9] The metagenomics RAST server - a public resource for the automatic phylogenetic and functional analysis of metagenomes [J].

Meyer, F. ;

Paarmann, D. ;

D'Souza, M. ;

Olson, R. ;

Glass, E. M. ;

Kubal, M. ;

Paczian, T. ;

Rodriguez, A. ;

Stevens, R. ;

Wilke, A. ;

Wilkening, J. ;

Edwards, R. A. .

BMC BIOINFORMATICS, 2008, 9 (1)

[10] Identifying biologically relevant differences between metagenomic communities [J].

Parks, Donovan H. ;

Beiko, Robert G. .

BIOINFORMATICS, 2010, 26 (06) :715-721

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