Expressed sequence tags from the phytopathogenic fungus Botrytis cinerea

被引：19

作者：

Viaud, M

Legeai, F

Pradier, JM

Brygoo, Y

Bitton, F

Weissenbach, J

Brunet-Simon, A

Duclert, A

Fillinger, S

Fortini, D

Gioti, A

Giraud, C

Halary, S

Lebrun, I

Le Pêcheur, P

Samson, D

Levis, C

机构：

[1] INRA, Unite PMDV, F-78086 Versailles, France

[2] INRA, URGI, F-91000 Evry, France

[3] CNRS, UMR 8030, F-91057 Evry, France

[4] INRA, UPMC, F-78086 Versailles, France

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2005年 / 111卷 / 02期

关键词：

cDNA; EST; grey mould;

D O I：

10.1007/s10658-004-1429-4

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A large set of genes was identified in the phytopathogenic fungus Botrytis cinerea by using an expressed sequence tag approach. The fungus was grown in axenic culture and a cDNA library was produced. From this library, 6559 ESTs were obtained. The combined sequences represent 3026 unisequences that corresponds to approximately one-quarter of the estimated total number of genes in B. cinerea. Approximately 18% of the ESTs showed significant similarities with genes coding for proteins with known functions,similar to56% were similar to genes coding for proteins with unknown functions and similar to26% were orphans. A substantial B. cinerea gene inventory including putative virulence factors was therefore obtained and is now available at the Genoplante-Info Database interface (http://urgi.infobiogen.fr///Projects/GPiDB/Interface/).

引用

页码：139 / 146

页数：8

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