Chrysanthemum boreale Makino Inhibits Oxidative Stress-Induced Neuronal Damage in Human Neuroblastoma SH-SY5Y Cells by Suppressing MAPK-Regulated Apoptosis

被引：4

作者：

Song, Parkyong ^{[1
]}

Choi, Seo Young ^{[2
]}

Hwang, Ji Sun ^{[3
]}

Park, Hyeon Cheal ^{[2
]}

Kim, Keun Ki ^{[2
]}

Son, Hong-Joo ^{[2
]}

Hong, Chang-Oh ^{[2
]}

Kim, Yu-Jin ^{[2
]}

Kim, Wanil ^{[4
]}

Lee, Kwang Min ^{[2
]}

机构：

[1] Pusan Natl Univ, Dept Convergence Med, Sch Med, Yangsan 50612, South Korea

[2] Pusan Natl Univ, Life & Ind Convergence Res Inst, Dept Life Sci & Environm Biochem, Miryang 50463, South Korea

[3] Daegu Gyeongbuk Med Innovat Fdn, New Drug Dev Ctr, K MEDI Hub, Daegu 41061, South Korea

[4] Gyeongsang Natl Univ, Inst Hlth Sci, Dept Biochem, Dept Convergence Med Sci,Coll Med, Jinju 52727, South Korea

来源：

MOLECULES | 2022年 / 27卷 / 17期

基金：

新加坡国家研究基金会;

关键词：

Chrysanthemum boreale Makino; oxidative stress; hydrogen peroxide; cell death; cell survival; neurodegenerative diseases; NF-KAPPA-B; ACTIVATED PROTEIN-KINASE; HYDROGEN-PEROXIDE; PC12; CELLS; PARKINSONS-DISEASE; SIGNALING PATHWAY; HEME OXYGENASE-1; ESSENTIAL OIL; IN-VITRO; CREB;

D O I：

10.3390/molecules27175498

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative stress has been demonstrated to play a pivotal role in the pathological processes of many neurodegenerative diseases. In the present study, we demonstrated that Chrysanthemum boreale Makino extract (CBME) suppresses oxidative stress-induced neurotoxicity in human neuroblastoma SH-SY5Y cells and elucidated the underlying molecular mechanism. Our observations revealed that CBME effectively protected neuronal cells against H2O2-induced cell death by preventing caspase-3 activation, Bax upregulation, Bcl-2 downregulation, activation of three mitogen-activated protein kinases (MAPKs), cAMP response element-binding protein (CREB) and NF-kappa B phosphorylation, and iNOS induction. These results provide evidence that CBME has remarkable neuroprotective properties in SH-SY5Y cells against oxidative damage, suggesting that the complementary or even alternative role of CBME in preventing and treating neurodegenerative diseases is worth further studies.

引用

页数：12

共 78 条

[1] Human ICE/CED-3 protease nomenclature [J].