Numerical and Structural Chromosomal Anomalies in Undifferentiated Pleomorphic Sarcoma

被引：0

作者：

Becerikli, Mustafa ^{[1
]}

Wieczorek, Stefan ^{[2
]}

Stricker, Ingo ^{[3
]}

Nambiar, Sandeep ^{[3
]}

Rittig, Andrea ^{[1
]}

Epplen, Joerg Thomas ^{[2
]}

Tannapfel, Andrea ^{[3
]}

Lehnhardt, Marcus ^{[1
]}

Steinstraesser, Lars ^{[1
]}

Jacobsen, Frank ^{[1
]}

机构：

[1] Ruhr Univ Bochum, BG Univ Hosp Bergmannsheil, Dept Plast Surg, Bochum, Germany

[2] Ruhr Univ Bochum, Dept Human Genet, Bochum, Germany

[3] Ruhr Univ Bochum, Inst Pathol, Bochum, Germany

来源：

ANTICANCER RESEARCH | 2014年 / 34卷 / 12期

关键词：

Chromosome aberrations; aneuploidy; gene amplification; sarcoma; fluorescent in situ hybridization; MALIGNANT FIBROUS HISTIOCYTOMA; SOFT-TISSUE SARCOMAS; GROWTH-FACTOR RECEPTOR; IN-SITU HYBRIDIZATION; GENE AMPLIFICATION; BREAST CARCINOMAS; CELLULAR-ORIGINS; SYNOVIAL SARCOMA; EWING SARCOMA; CANCER;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Malignant fibrous histiocytoma (MFH) or undifferentiated pleomorphic sarcoma (UPS) is the most common soft-tissue sarcoma of late adult life. Further advances in genetic characterization are warranted. The aim of this study. was to search for numerical and structural chromosomal anomalies in UPS. Materials and Methods: We investigated five sarcoma-specific chromosomal translocations, five oncogene amplifications as well as the numerical karyotype of 19 UPS samples and one UPS/MFH cell line (U2197) using FISH probes on interphase nuclei. Results: Our results demonstrate that chromosomal translocations involving CHOP, SYT, EWS, FUS and FKHR genes are absent. Furthermore, amplification of ERBB2 (10.5%) and MDM2 (10.5%) was observed whereas the EGFR, C-MYC and N-MYC genes were not amplified. Interestingly, predominant aneuploidies were found in eight chromosomes. Conclusion: The data demonstrate rarity of sarcoma-specific chromosomal breaks and onco gene amplifications in UPS, yet polysomic chromosomes appear more characteristically in this condition.

引用

页码：7119 / 7127

页数：9

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