Raman spectroscopy methods for detecting and imaging supported lipid bilayers

被引：13
|
作者：
Sweetenham, Claire S. [1 ]
Notingher, Ioan [1 ]
机构：
[1] Univ Nottingham, Sch Phys & Astron, Nanosci Grp, Nottingham NG7 2RD, England
来源：
SPECTROSCOPY-AN INTERNATIONAL JOURNAL | 2010年 / 24卷 / 1-2期
关键词：
Supported lipid bilayers; atomic force microscopy; Raman microspectroscopy; surface-enhanced Raman spectroscopy; FILMS;
D O I：
10.1155/2010/273028
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a Raman microspectroseopy system optimised for studying supported lipid bilayers (SLB). This system combines the benefits of Raman spectroscopy with the high spatial resolution of confocal microscopy. Furthermore. the additional incorporation of an atomic force microscope (AFM) makes it possible to directly correlate chemical information with spatial features of samples at the nanoscale. We focus on the limits of this system for detecting a single SLB and imaging its microdomains, and employ surface-enhanced Raman spectroscopy (SERS) to improve the sensitivity achieved with Raman microspectroscopy.
引用
收藏
页码：113 / 117
页数：5
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